Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y.
Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here, we describe the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their messenger RNAs are found in certain...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2010
|
| _version_ | 1797062175953518592 |
|---|---|
| author | Wiedemann, S Mildner, SN Bönisch, C Israel, L Maiser, A Matheisl, S Straub, T Merkl, R Leonhardt, H Kremmer, E Schermelleh, L Hake, S |
| author_facet | Wiedemann, S Mildner, SN Bönisch, C Israel, L Maiser, A Matheisl, S Straub, T Merkl, R Leonhardt, H Kremmer, E Schermelleh, L Hake, S |
| author_sort | Wiedemann, S |
| collection | OXFORD |
| description | Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here, we describe the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their messenger RNAs are found in certain human cell lines, in addition to several normal and malignant human tissues. In keeping with their primate specificity, H3.X and H3.Y are detected in different brain regions. Transgenic H3.X and H3.Y proteins are stably incorporated into chromatin in a similar fashion to the known H3 variants. Importantly, we demonstrate biochemically and by mass spectrometry that endogenous H3.Y protein exists in vivo, and that stress stimuli, such as starvation and cellular density, increase the abundance of H3.Y-expressing cells. Global transcriptome analysis revealed that knockdown of H3.Y affects cell growth and leads to changes in the expression of many genes involved in cell cycle control. Thus, H3.Y is a novel histone variant involved in the regulation of cellular responses to outside stimuli. |
| first_indexed | 2024-03-06T20:41:49Z |
| format | Journal article |
| id | oxford-uuid:348cacff-947f-43e9-9bbc-819652ff78e7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:41:49Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:348cacff-947f-43e9-9bbc-819652ff78e72022-03-26T13:26:38ZIdentification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:348cacff-947f-43e9-9bbc-819652ff78e7EnglishSymplectic Elements at Oxford2010Wiedemann, SMildner, SNBönisch, CIsrael, LMaiser, AMatheisl, SStraub, TMerkl, RLeonhardt, HKremmer, ESchermelleh, LHake, SNucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here, we describe the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their messenger RNAs are found in certain human cell lines, in addition to several normal and malignant human tissues. In keeping with their primate specificity, H3.X and H3.Y are detected in different brain regions. Transgenic H3.X and H3.Y proteins are stably incorporated into chromatin in a similar fashion to the known H3 variants. Importantly, we demonstrate biochemically and by mass spectrometry that endogenous H3.Y protein exists in vivo, and that stress stimuli, such as starvation and cellular density, increase the abundance of H3.Y-expressing cells. Global transcriptome analysis revealed that knockdown of H3.Y affects cell growth and leads to changes in the expression of many genes involved in cell cycle control. Thus, H3.Y is a novel histone variant involved in the regulation of cellular responses to outside stimuli. |
| spellingShingle | Wiedemann, S Mildner, SN Bönisch, C Israel, L Maiser, A Matheisl, S Straub, T Merkl, R Leonhardt, H Kremmer, E Schermelleh, L Hake, S Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title | Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title_full | Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title_fullStr | Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title_full_unstemmed | Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title_short | Identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. |
| title_sort | identification and characterization of two novel primate specific histone h3 variants h3 x and h3 y |
| work_keys_str_mv | AT wiedemanns identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT mildnersn identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT bonischc identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT israell identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT maisera identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT matheisls identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT straubt identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT merklr identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT leonhardth identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT kremmere identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT schermellehl identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y AT hakes identificationandcharacterizationoftwonovelprimatespecifichistoneh3variantsh3xandh3y |