Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?
We have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1797062185342468096 |
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| author | Ashfield, R Enriquez-Harris, P Proudfoot, N |
| author_facet | Ashfield, R Enriquez-Harris, P Proudfoot, N |
| author_sort | Ashfield, R |
| collection | OXFORD |
| description | We have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally similar sequence downstream of the human alpha 2 globin gene reveals that both signals function in an orientation dependent manner, with subfragments of the whole signal displaying partial effects. In the case of the C2 termination sequence a protein binds within it, and is partially responsible for the termination effect. We further demonstrate that the same (or closely related) protein binds to the ME1a1 site in the murine c-myc promoter, which has been implicated in c-myc attenuation. We suggest that the termination/pause sequences positioned downstream of a gene's poly(A) site may constitute the general signals that elicit transcriptional termination in genes transcribed by RNA polymerase II. |
| first_indexed | 2024-03-06T20:41:58Z |
| format | Journal article |
| id | oxford-uuid:3496f4b3-cad9-41c8-b195-a26cf090387a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:41:58Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:3496f4b3-cad9-41c8-b195-a26cf090387a2022-03-26T13:26:51ZTranscriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3496f4b3-cad9-41c8-b195-a26cf090387aEnglishSymplectic Elements at Oxford1991Ashfield, REnriquez-Harris, PProudfoot, NWe have demonstrated, using a combination of nuclear run-off and poly(A) site competition assays, that transcriptional termination occurs between the closely spaced human complement genes, C2 and Factor B, soon after the C2 poly(A) site. A comparison of the C2 termination signal with a functionally similar sequence downstream of the human alpha 2 globin gene reveals that both signals function in an orientation dependent manner, with subfragments of the whole signal displaying partial effects. In the case of the C2 termination sequence a protein binds within it, and is partially responsible for the termination effect. We further demonstrate that the same (or closely related) protein binds to the ME1a1 site in the murine c-myc promoter, which has been implicated in c-myc attenuation. We suggest that the termination/pause sequences positioned downstream of a gene's poly(A) site may constitute the general signals that elicit transcriptional termination in genes transcribed by RNA polymerase II. |
| spellingShingle | Ashfield, R Enriquez-Harris, P Proudfoot, N Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title | Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title_full | Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title_fullStr | Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title_full_unstemmed | Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title_short | Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc? |
| title_sort | transcriptional termination between the closely linked human complement genes c2 and factor b common termination factor for c2 and c myc |
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