The biochemistry of antigen presentation
<p>This thesis describes studies on the binding of peptides to the murine major histocompatibility complex (MHC) class I molecule H-2D<sup>b</sup> (D<sup>b</sup>). The expression of the recombinant soluble D<sup>b</sup> molecule in Chinese hamster ovary cel...
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| Format: | Thesis |
| Language: | English |
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1996
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| _version_ | 1817932683287724032 |
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| author | Springer, S Springer, Sebastian Hartmut |
| author_facet | Springer, S Springer, Sebastian Hartmut |
| author_sort | Springer, S |
| collection | OXFORD |
| description | <p>This thesis describes studies on the binding of peptides to the murine major histocompatibility complex (MHC) class I molecule H-2D<sup>b</sup> (D<sup>b</sup>). The expression of the recombinant soluble D<sup>b</sup> molecule in Chinese hamster ovary cells and its subsequent purification by nickel affinity chromatography, gel filtration, and preparative native isoelectric focusing are reported. The product is the correct molecule, homogeneous, a dimer of dimers, and free of endogenous peptide.</p> <p>A novel binding assay based on the enhancement of natural tryptophan fluorescence by the binding of peptide is introduced. This assay is used to determine melting curves of the empty and peptide-loaded protein, and to measure association rate constants by stopped-flow fluorescence spectroscopy.</p> <p>Radioligand binding measurements of equilibrium as well as association and dissociation rate constants and their temperature dependence are reported. In agreement with earlier observations, the ratio of association and dissociation rate constants is much larger than the equilibrium association constant.</p> <p>Fluorescence anisotropy decay spectroscopy gives evidence for conformational alterations in the D<sup>b</sup> molecule upon peptide binding.</p> <p>The data, possible errors and ways to avoid them, and mathematical models of binding are discussed to obtain an overall picture of the binding process.</p> |
| first_indexed | 2024-03-06T20:42:48Z |
| format | Thesis |
| id | oxford-uuid:34d1afd2-fafc-4732-8e43-e00dcd8460d1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:41:48Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:34d1afd2-fafc-4732-8e43-e00dcd8460d12024-12-07T12:58:56ZThe biochemistry of antigen presentationThesishttp://purl.org/coar/resource_type/c_db06uuid:34d1afd2-fafc-4732-8e43-e00dcd8460d1Antigen presenting cellsPeptidesMajor histocompatibility complexEnglishPolonsky Theses Digitisation Project1996Springer, SSpringer, Sebastian Hartmut<p>This thesis describes studies on the binding of peptides to the murine major histocompatibility complex (MHC) class I molecule H-2D<sup>b</sup> (D<sup>b</sup>). The expression of the recombinant soluble D<sup>b</sup> molecule in Chinese hamster ovary cells and its subsequent purification by nickel affinity chromatography, gel filtration, and preparative native isoelectric focusing are reported. The product is the correct molecule, homogeneous, a dimer of dimers, and free of endogenous peptide.</p> <p>A novel binding assay based on the enhancement of natural tryptophan fluorescence by the binding of peptide is introduced. This assay is used to determine melting curves of the empty and peptide-loaded protein, and to measure association rate constants by stopped-flow fluorescence spectroscopy.</p> <p>Radioligand binding measurements of equilibrium as well as association and dissociation rate constants and their temperature dependence are reported. In agreement with earlier observations, the ratio of association and dissociation rate constants is much larger than the equilibrium association constant.</p> <p>Fluorescence anisotropy decay spectroscopy gives evidence for conformational alterations in the D<sup>b</sup> molecule upon peptide binding.</p> <p>The data, possible errors and ways to avoid them, and mathematical models of binding are discussed to obtain an overall picture of the binding process.</p> |
| spellingShingle | Antigen presenting cells Peptides Major histocompatibility complex Springer, S Springer, Sebastian Hartmut The biochemistry of antigen presentation |
| title | The biochemistry of antigen presentation |
| title_full | The biochemistry of antigen presentation |
| title_fullStr | The biochemistry of antigen presentation |
| title_full_unstemmed | The biochemistry of antigen presentation |
| title_short | The biochemistry of antigen presentation |
| title_sort | biochemistry of antigen presentation |
| topic | Antigen presenting cells Peptides Major histocompatibility complex |
| work_keys_str_mv | AT springers thebiochemistryofantigenpresentation AT springersebastianhartmut thebiochemistryofantigenpresentation AT springers biochemistryofantigenpresentation AT springersebastianhartmut biochemistryofantigenpresentation |