'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome.
B lymphocytes expressing CD5 (CD5+B cells) and T lymphocytes using the gamma and delta chains to form their antigen receptor (gamma delta +T cells) are major populations in developing fetuses, but become relatively minor in normal adults. However, both subsets are expanded in the peripheral blood of...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1989
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| _version_ | 1797062358210707456 |
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| author | Plater-Zyberk, C Brennan, F Feldmann, M Maini, R |
| author_facet | Plater-Zyberk, C Brennan, F Feldmann, M Maini, R |
| author_sort | Plater-Zyberk, C |
| collection | OXFORD |
| description | B lymphocytes expressing CD5 (CD5+B cells) and T lymphocytes using the gamma and delta chains to form their antigen receptor (gamma delta +T cells) are major populations in developing fetuses, but become relatively minor in normal adults. However, both subsets are expanded in the peripheral blood of more than 50% of patients with rheumatoid arthritis and primary Sjögren's syndrome. We have examined the surface phenotype of these subsets using flow cytometry and have studied the frequency of IgM-producing lines after EBV-transformation of sorted CD5+B and CD5-B cells isolated from neonatal umbilical vein and RA peripheral blood. The intensity of CD5 expression on B cells was at least 10 times 'duller' than on T cells, CD5 'dull' cells were CD3 negative, and T cells bearing the gamma delta antigen receptor did not express either CD4 or CD8 on their surface. In vitro stimulation by Staphylococcus aureus Cowan I or transformation by Epstein-Barr virus of CD5+B cells resulted in loss of CD5 antigen from the surface of B cells. EBV-transformation of sorted CD5+B and CD5-B lymphocytes from neonatal blood gave rise to IgM-secretion in 100% of the Ig-secreting lines. CD5+B fraction isolated from RA blood also generated 100% IgM-secreting lines, whereas 29% of the Ig-secreting lines obtained from RA CD5-B fraction did not secrete IgM. The function of these 'fetal-type' T and B lymphocytes is unknown, however their expansion in rheumatoid arthritis and primary Sjögren's Syndrome suggests that they may play a role in autoimmune diseases. |
| first_indexed | 2024-03-06T20:44:24Z |
| format | Journal article |
| id | oxford-uuid:355d9916-8447-4103-8ea8-c0d28159ebbc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:44:24Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:355d9916-8447-4103-8ea8-c0d28159ebbc2022-03-26T13:31:37Z'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:355d9916-8447-4103-8ea8-c0d28159ebbcEnglishSymplectic Elements at Oxford1989Plater-Zyberk, CBrennan, FFeldmann, MMaini, RB lymphocytes expressing CD5 (CD5+B cells) and T lymphocytes using the gamma and delta chains to form their antigen receptor (gamma delta +T cells) are major populations in developing fetuses, but become relatively minor in normal adults. However, both subsets are expanded in the peripheral blood of more than 50% of patients with rheumatoid arthritis and primary Sjögren's syndrome. We have examined the surface phenotype of these subsets using flow cytometry and have studied the frequency of IgM-producing lines after EBV-transformation of sorted CD5+B and CD5-B cells isolated from neonatal umbilical vein and RA peripheral blood. The intensity of CD5 expression on B cells was at least 10 times 'duller' than on T cells, CD5 'dull' cells were CD3 negative, and T cells bearing the gamma delta antigen receptor did not express either CD4 or CD8 on their surface. In vitro stimulation by Staphylococcus aureus Cowan I or transformation by Epstein-Barr virus of CD5+B cells resulted in loss of CD5 antigen from the surface of B cells. EBV-transformation of sorted CD5+B and CD5-B lymphocytes from neonatal blood gave rise to IgM-secretion in 100% of the Ig-secreting lines. CD5+B fraction isolated from RA blood also generated 100% IgM-secreting lines, whereas 29% of the Ig-secreting lines obtained from RA CD5-B fraction did not secrete IgM. The function of these 'fetal-type' T and B lymphocytes is unknown, however their expansion in rheumatoid arthritis and primary Sjögren's Syndrome suggests that they may play a role in autoimmune diseases. |
| spellingShingle | Plater-Zyberk, C Brennan, F Feldmann, M Maini, R 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title | 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title_full | 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title_fullStr | 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title_full_unstemmed | 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title_short | 'Fetal-type' B and T lymphocytes in rheumatoid arthritis and primary Sjogren's syndrome. |
| title_sort | fetal type b and t lymphocytes in rheumatoid arthritis and primary sjogren s syndrome |
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