Diverse Durham collection phages demonstrate complex BREX defence responses
Bacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations make them attractive alternative antibacterials for use in combatting the rise in antimicrobial resistance. This effort may be hindered due t...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Society for Microbiology
2023
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| _version_ | 1826311611260862464 |
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| author | Kelly, A Went, SC Mariano, G Shaw, L Picton, DM Duffner, SJ Coates, I Herdman-Grant, R Gordeeva, J Drobiazko, A Isaev, A Lee, Y-J Lutyen, Y Morgan, RD Weigele, P Severinov, K Wenner, N Hinton, JCD Blower, TR |
| author_facet | Kelly, A Went, SC Mariano, G Shaw, L Picton, DM Duffner, SJ Coates, I Herdman-Grant, R Gordeeva, J Drobiazko, A Isaev, A Lee, Y-J Lutyen, Y Morgan, RD Weigele, P Severinov, K Wenner, N Hinton, JCD Blower, TR |
| author_sort | Kelly, A |
| collection | OXFORD |
| description | Bacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations make them attractive alternative antibacterials for use in combatting the rise in antimicrobial resistance. This effort may be hindered due to bacterial defences such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be understood about bacteria-phage interactions and the outcomes of bacterial phage defence. Here, we present the annotated genomes of twelve novel bacteriophage species isolated from water sources in Durham, UK, during undergraduate practical classes. The collection includes diverse species from across known phylogenetic groups. Comparative analyses of two novel phages from the collection suggests they may be founding members of a new genus. Using this Durham phage collection we determined that particular BREX defence systems were likely to confer a varied degree of resistance against an invading phage. We concluded that the number of BREX target motifs encoded in the phage genome was not proportional to the degree of susceptibility. |
| first_indexed | 2024-03-07T08:12:20Z |
| format | Journal article |
| id | oxford-uuid:35a1dc70-9fb2-4110-8992-128f59028ee1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:12:20Z |
| publishDate | 2023 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:35a1dc70-9fb2-4110-8992-128f59028ee12023-12-11T09:52:16ZDiverse Durham collection phages demonstrate complex BREX defence responsesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:35a1dc70-9fb2-4110-8992-128f59028ee1EnglishSymplectic ElementsAmerican Society for Microbiology2023Kelly, AWent, SCMariano, GShaw, LPicton, DMDuffner, SJCoates, IHerdman-Grant, RGordeeva, JDrobiazko, AIsaev, ALee, Y-JLutyen, YMorgan, RDWeigele, PSeverinov, KWenner, NHinton, JCDBlower, TRBacteriophages (phages) outnumber bacteria ten-to-one and cause infections at a rate of 1025 per second. The ability of phages to reduce bacterial populations make them attractive alternative antibacterials for use in combatting the rise in antimicrobial resistance. This effort may be hindered due to bacterial defences such as Bacteriophage Exclusion (BREX) that have arisen from the constant evolutionary battle between bacteria and phages. For phages to be widely accepted as therapeutics in Western medicine, more must be understood about bacteria-phage interactions and the outcomes of bacterial phage defence. Here, we present the annotated genomes of twelve novel bacteriophage species isolated from water sources in Durham, UK, during undergraduate practical classes. The collection includes diverse species from across known phylogenetic groups. Comparative analyses of two novel phages from the collection suggests they may be founding members of a new genus. Using this Durham phage collection we determined that particular BREX defence systems were likely to confer a varied degree of resistance against an invading phage. We concluded that the number of BREX target motifs encoded in the phage genome was not proportional to the degree of susceptibility. |
| spellingShingle | Kelly, A Went, SC Mariano, G Shaw, L Picton, DM Duffner, SJ Coates, I Herdman-Grant, R Gordeeva, J Drobiazko, A Isaev, A Lee, Y-J Lutyen, Y Morgan, RD Weigele, P Severinov, K Wenner, N Hinton, JCD Blower, TR Diverse Durham collection phages demonstrate complex BREX defence responses |
| title | Diverse Durham collection phages demonstrate complex BREX defence responses |
| title_full | Diverse Durham collection phages demonstrate complex BREX defence responses |
| title_fullStr | Diverse Durham collection phages demonstrate complex BREX defence responses |
| title_full_unstemmed | Diverse Durham collection phages demonstrate complex BREX defence responses |
| title_short | Diverse Durham collection phages demonstrate complex BREX defence responses |
| title_sort | diverse durham collection phages demonstrate complex brex defence responses |
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