Electroformation of giant unilamellar vesicles on stainless steel electrodes
Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relative...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
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American Chemical Society
2017
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| _version_ | 1797062458940063744 |
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| author | Pereno, V Carugo, D Bau, L Sezgin, E de la Serna, J Eggeling, C Stride, E |
| author_facet | Pereno, V Carugo, D Bau, L Sezgin, E de la Serna, J Eggeling, C Stride, E |
| author_sort | Pereno, V |
| collection | OXFORD |
| description | Giant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively mono-disperse, defect-free vesicles. Currently, however, it is relatively expensive and time-consuming compared to other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis. |
| first_indexed | 2024-03-06T20:45:50Z |
| format | Journal article |
| id | oxford-uuid:35dac811-ab92-4b12-aaa7-ee38fb22c1a1 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:45:50Z |
| publishDate | 2017 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:35dac811-ab92-4b12-aaa7-ee38fb22c1a12022-03-26T13:34:27ZElectroformation of giant unilamellar vesicles on stainless steel electrodesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:35dac811-ab92-4b12-aaa7-ee38fb22c1a1Symplectic Elements at OxfordAmerican Chemical Society2017Pereno, VCarugo, DBau, LSezgin, Ede la Serna, JEggeling, CStride, EGiant unilamellar vesicles (GUVs) are well-established model systems for studying membrane structure and dynamics. Electroformation, also referred to as electro-swelling, is one of the most prevalent methods for producing GUVs, as it enables modulation of the lipid hydration process to form relatively mono-disperse, defect-free vesicles. Currently, however, it is relatively expensive and time-consuming compared to other methods. In this study, we demonstrate that 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) GUVs can be prepared readily at a fraction of the cost on stainless steel electrodes such as commercially available syringe needles, without any evidence of lipid oxidation or hydrolysis. |
| spellingShingle | Pereno, V Carugo, D Bau, L Sezgin, E de la Serna, J Eggeling, C Stride, E Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title | Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title_full | Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title_fullStr | Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title_full_unstemmed | Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title_short | Electroformation of giant unilamellar vesicles on stainless steel electrodes |
| title_sort | electroformation of giant unilamellar vesicles on stainless steel electrodes |
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