| Summary: | A total of 147 NANOG and NANOGNB Refseq, Ensembl and blastp identified un-annotated protein sequences from mammals, reptiles, birds, and fish were downloaded and aligned using MAFFT before being visually inspected for conserved motifs. A series of six conserved motifs were identified.
1 - Alignment positions 505-519, this region of the NANOG protein is conserved between all species but not shared with NANOGNB.
2 - Alignment positions 524-530, this region of the NANOG and NANOGNB proteins is conserved across all species .
3 - Alignment positions 544-554, this region of NANOGNB is conserved only between reptile and bird NANOG proteins, and not with the mammal NANOG proteins.
4 - Alignment positions 555-581, this region is conserved between the NANOGNB proteins.
5 - Alignment positions 584-650, this region defines the homeodomain and is conserved across all NANOG and NANOGNB proteins.
6 - Alignment positions 684-690, this region of the NANOG protein is conserved between all species but not shared with NANOGNB.
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