The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk.
BACKGROUND: The risk of postnatal HIV transmission is associated with the magnitude of the milk virus load. While HIV-specific cellular immune responses control systemic virus load and are detectable in milk, the contribution of these responses to the control of virus load in milk is unknown. METHO...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Public Library of Science
2011
|
| _version_ | 1797062705114251264 |
|---|---|
| author | Mahlokozera, T Kang, H Goonetilleke, N Stacey, A Lovingood, R Denny, T Kalilani, L Bunn, J Meshnick, SR Borrow, P Letvin, N Permar, SR |
| author_facet | Mahlokozera, T Kang, H Goonetilleke, N Stacey, A Lovingood, R Denny, T Kalilani, L Bunn, J Meshnick, SR Borrow, P Letvin, N Permar, SR |
| author_sort | Mahlokozera, T |
| collection | OXFORD |
| description | BACKGROUND: The risk of postnatal HIV transmission is associated with the magnitude of the milk virus load. While HIV-specific cellular immune responses control systemic virus load and are detectable in milk, the contribution of these responses to the control of virus load in milk is unknown. METHODS: We assessed the magnitude of the immunodominant GagRY11 and subdominant EnvKY9-specific CD8+ T lymphocyte response in blood and milk of 10 A*3002+, HIV-infected Malawian women throughout the period of lactation and correlated this response to milk virus RNA load and markers of breast inflammation. RESULTS: The magnitude and kinetics of the HIV-specific CD8+ T lymphocyte responses were discordant in blood and milk of the right and left breast, indicating independent regulation of these responses in each breast. However, there was no correlation between the magnitude of the HIV-specific CD8+ T lymphocyte response and the milk virus RNA load. Further, there was no correlation between the magnitude of this response and markers of breast inflammation. CONCLUSIONS: The magnitude of the HIV-specific CD8+ T lymphocyte response in milk does not appear to be solely determined by the milk virus RNA load and is likely only one of the factors contributing to maintenance of low virus load in milk. |
| first_indexed | 2024-03-06T20:49:23Z |
| format | Journal article |
| id | oxford-uuid:370f63e3-e3de-4a0c-935f-9f28235bae29 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:49:23Z |
| publishDate | 2011 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:370f63e3-e3de-4a0c-935f-9f28235bae292022-03-26T13:41:39ZThe magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:370f63e3-e3de-4a0c-935f-9f28235bae29EnglishSymplectic Elements at OxfordPublic Library of Science2011Mahlokozera, TKang, HGoonetilleke, NStacey, ALovingood, RDenny, TKalilani, LBunn, JMeshnick, SRBorrow, PLetvin, NPermar, SR BACKGROUND: The risk of postnatal HIV transmission is associated with the magnitude of the milk virus load. While HIV-specific cellular immune responses control systemic virus load and are detectable in milk, the contribution of these responses to the control of virus load in milk is unknown. METHODS: We assessed the magnitude of the immunodominant GagRY11 and subdominant EnvKY9-specific CD8+ T lymphocyte response in blood and milk of 10 A*3002+, HIV-infected Malawian women throughout the period of lactation and correlated this response to milk virus RNA load and markers of breast inflammation. RESULTS: The magnitude and kinetics of the HIV-specific CD8+ T lymphocyte responses were discordant in blood and milk of the right and left breast, indicating independent regulation of these responses in each breast. However, there was no correlation between the magnitude of the HIV-specific CD8+ T lymphocyte response and the milk virus RNA load. Further, there was no correlation between the magnitude of this response and markers of breast inflammation. CONCLUSIONS: The magnitude of the HIV-specific CD8+ T lymphocyte response in milk does not appear to be solely determined by the milk virus RNA load and is likely only one of the factors contributing to maintenance of low virus load in milk. |
| spellingShingle | Mahlokozera, T Kang, H Goonetilleke, N Stacey, A Lovingood, R Denny, T Kalilani, L Bunn, J Meshnick, SR Borrow, P Letvin, N Permar, SR The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title | The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title_full | The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title_fullStr | The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title_full_unstemmed | The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title_short | The magnitude and kinetics of the mucosal HIV-specific CD8+ T lymphocyte response and virus RNA load in breast milk. |
| title_sort | magnitude and kinetics of the mucosal hiv specific cd8 t lymphocyte response and virus rna load in breast milk |
| work_keys_str_mv | AT mahlokozerat themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT kangh themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT goonetilleken themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT staceya themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT lovingoodr themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT dennyt themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT kalilanil themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT bunnj themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT meshnicksr themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT borrowp themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT letvinn themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT permarsr themagnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT mahlokozerat magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT kangh magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT goonetilleken magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT staceya magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT lovingoodr magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT dennyt magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT kalilanil magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT bunnj magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT meshnicksr magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT borrowp magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT letvinn magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk AT permarsr magnitudeandkineticsofthemucosalhivspecificcd8tlymphocyteresponseandvirusrnaloadinbreastmilk |