Site of attachment of retinal in bacteriorhodopsin.
The identity of the lysine residue in bacteriorhodopsin to which the chromophore, retinal, is attached as a Schiff's base has been reinvestigated. Retinal is now shown to be linked to lysine-216 and not lysine-41, as had been concluded previously. The retinal in purple membrane was replaced by...
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
1981
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| _version_ | 1826267240253620224 |
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| author | Bayley, H Huang, K Radhakrishnan, R Ross, A Takagaki, Y Khorana, H |
| author_facet | Bayley, H Huang, K Radhakrishnan, R Ross, A Takagaki, Y Khorana, H |
| author_sort | Bayley, H |
| collection | OXFORD |
| description | The identity of the lysine residue in bacteriorhodopsin to which the chromophore, retinal, is attached as a Schiff's base has been reinvestigated. Retinal is now shown to be linked to lysine-216 and not lysine-41, as had been concluded previously. The retinal in purple membrane was replaced by [15-3H]retinal, the Schiff's base linkage was reduced with NaBH4 while the sample was illuminated, and the resulting [retinyl-3H]bacterio-opsin was cleaved with CNBr. The radioactivity was present exclusively in the COOH-terminal peptide (amino acids 210--248). Sequence analysis showed that the [3H]retinal was attached to lysine-216. The same site was labeled when purple membrane was reduced with NaBH4 in the light at pH greater than 10 or at pH 8 and when membranes modified with ether or solubilized with hexadecyltrimethylammonium bromide were reduced in the dark. Our finding places of Schiff's base linkage close to the midpoint of the putative membrane-spanning alpha-helix that is directly connected to the COOH terminus of bacteriorhodopsin. |
| first_indexed | 2024-03-06T20:51:09Z |
| format | Journal article |
| id | oxford-uuid:379f0f9e-0e05-4063-b5f7-29ad22424e2d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:51:09Z |
| publishDate | 1981 |
| record_format | dspace |
| spelling | oxford-uuid:379f0f9e-0e05-4063-b5f7-29ad22424e2d2022-03-26T13:45:03ZSite of attachment of retinal in bacteriorhodopsin.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:379f0f9e-0e05-4063-b5f7-29ad22424e2dEnglishSymplectic Elements at Oxford1981Bayley, HHuang, KRadhakrishnan, RRoss, ATakagaki, YKhorana, HThe identity of the lysine residue in bacteriorhodopsin to which the chromophore, retinal, is attached as a Schiff's base has been reinvestigated. Retinal is now shown to be linked to lysine-216 and not lysine-41, as had been concluded previously. The retinal in purple membrane was replaced by [15-3H]retinal, the Schiff's base linkage was reduced with NaBH4 while the sample was illuminated, and the resulting [retinyl-3H]bacterio-opsin was cleaved with CNBr. The radioactivity was present exclusively in the COOH-terminal peptide (amino acids 210--248). Sequence analysis showed that the [3H]retinal was attached to lysine-216. The same site was labeled when purple membrane was reduced with NaBH4 in the light at pH greater than 10 or at pH 8 and when membranes modified with ether or solubilized with hexadecyltrimethylammonium bromide were reduced in the dark. Our finding places of Schiff's base linkage close to the midpoint of the putative membrane-spanning alpha-helix that is directly connected to the COOH terminus of bacteriorhodopsin. |
| spellingShingle | Bayley, H Huang, K Radhakrishnan, R Ross, A Takagaki, Y Khorana, H Site of attachment of retinal in bacteriorhodopsin. |
| title | Site of attachment of retinal in bacteriorhodopsin. |
| title_full | Site of attachment of retinal in bacteriorhodopsin. |
| title_fullStr | Site of attachment of retinal in bacteriorhodopsin. |
| title_full_unstemmed | Site of attachment of retinal in bacteriorhodopsin. |
| title_short | Site of attachment of retinal in bacteriorhodopsin. |
| title_sort | site of attachment of retinal in bacteriorhodopsin |
| work_keys_str_mv | AT bayleyh siteofattachmentofretinalinbacteriorhodopsin AT huangk siteofattachmentofretinalinbacteriorhodopsin AT radhakrishnanr siteofattachmentofretinalinbacteriorhodopsin AT rossa siteofattachmentofretinalinbacteriorhodopsin AT takagakiy siteofattachmentofretinalinbacteriorhodopsin AT khoranah siteofattachmentofretinalinbacteriorhodopsin |