Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.
Erythropoietin, the major hormone controlling red-cell production, is regulated in part through oxygen-dependent changes in the rate of transcription of its gene. Using transient transfection in HepG2 cells, we have defined a DNA sequence, located 120 base pairs 3' to the poly(A)-addition site...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1991
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| _version_ | 1797062885514412032 |
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| author | Pugh, C Tan, C Jones, R Ratcliffe, P |
| author_facet | Pugh, C Tan, C Jones, R Ratcliffe, P |
| author_sort | Pugh, C |
| collection | OXFORD |
| description | Erythropoietin, the major hormone controlling red-cell production, is regulated in part through oxygen-dependent changes in the rate of transcription of its gene. Using transient transfection in HepG2 cells, we have defined a DNA sequence, located 120 base pairs 3' to the poly(A)-addition site of the mouse erythropoietin gene, that confers oxygen-regulated expression on a variety of heterologous promoters. The sequence has the typical features of a eukaryotic enhancer. Approximately 70 base pairs are necessary for full activity, but reiteration restores activity to shorter inactive sequences. This enhancer operates in HepG2 and Hep3B cells, but not in Chinese hamster ovary cells or mouse erythroleukemia cells, and responds to cobalt but not to cyanide or 2-deoxyglucose, thus reflecting the physiological control of erythropoietin production accurately. |
| first_indexed | 2024-03-06T20:52:01Z |
| format | Journal article |
| id | oxford-uuid:37e95d29-5d58-4daf-bc9b-163a64d94b14 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:52:01Z |
| publishDate | 1991 |
| record_format | dspace |
| spelling | oxford-uuid:37e95d29-5d58-4daf-bc9b-163a64d94b142022-03-26T13:46:46ZFunctional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:37e95d29-5d58-4daf-bc9b-163a64d94b14EnglishSymplectic Elements at Oxford1991Pugh, CTan, CJones, RRatcliffe, PErythropoietin, the major hormone controlling red-cell production, is regulated in part through oxygen-dependent changes in the rate of transcription of its gene. Using transient transfection in HepG2 cells, we have defined a DNA sequence, located 120 base pairs 3' to the poly(A)-addition site of the mouse erythropoietin gene, that confers oxygen-regulated expression on a variety of heterologous promoters. The sequence has the typical features of a eukaryotic enhancer. Approximately 70 base pairs are necessary for full activity, but reiteration restores activity to shorter inactive sequences. This enhancer operates in HepG2 and Hep3B cells, but not in Chinese hamster ovary cells or mouse erythroleukemia cells, and responds to cobalt but not to cyanide or 2-deoxyglucose, thus reflecting the physiological control of erythropoietin production accurately. |
| spellingShingle | Pugh, C Tan, C Jones, R Ratcliffe, P Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title | Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title_full | Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title_fullStr | Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title_full_unstemmed | Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title_short | Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene. |
| title_sort | functional analysis of an oxygen regulated transcriptional enhancer lying 3 to the mouse erythropoietin gene |
| work_keys_str_mv | AT pughc functionalanalysisofanoxygenregulatedtranscriptionalenhancerlying3tothemouseerythropoietingene AT tanc functionalanalysisofanoxygenregulatedtranscriptionalenhancerlying3tothemouseerythropoietingene AT jonesr functionalanalysisofanoxygenregulatedtranscriptionalenhancerlying3tothemouseerythropoietingene AT ratcliffep functionalanalysisofanoxygenregulatedtranscriptionalenhancerlying3tothemouseerythropoietingene |