Aberration-free optical refocusing in high numerical aperture microscopy.
We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtain...
| Egile Nagusiak: | , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
2007
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| _version_ | 1826267365585715200 |
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| author | Botcherby, E Juskaitis, R Booth, M Wilson, T |
| author_facet | Botcherby, E Juskaitis, R Booth, M Wilson, T |
| author_sort | Botcherby, E |
| collection | OXFORD |
| description | We describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 mum for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen. |
| first_indexed | 2024-03-06T20:53:05Z |
| format | Journal article |
| id | oxford-uuid:383f7173-d481-406e-9bf0-dd7117048798 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:53:05Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:383f7173-d481-406e-9bf0-dd71170487982022-03-26T13:48:56ZAberration-free optical refocusing in high numerical aperture microscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:383f7173-d481-406e-9bf0-dd7117048798EnglishSymplectic Elements at Oxford2007Botcherby, EJuskaitis, RBooth, MWilson, TWe describe a method of optical refocusing for high numerical aperture (NA) systems that is particularly relevant for confocal and multiphoton microscopy. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free images can be obtained over an axial scan range of 70 mum for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method will enable high axial scan speeds without mechanical interference between the objective lens and the specimen. |
| spellingShingle | Botcherby, E Juskaitis, R Booth, M Wilson, T Aberration-free optical refocusing in high numerical aperture microscopy. |
| title | Aberration-free optical refocusing in high numerical aperture microscopy. |
| title_full | Aberration-free optical refocusing in high numerical aperture microscopy. |
| title_fullStr | Aberration-free optical refocusing in high numerical aperture microscopy. |
| title_full_unstemmed | Aberration-free optical refocusing in high numerical aperture microscopy. |
| title_short | Aberration-free optical refocusing in high numerical aperture microscopy. |
| title_sort | aberration free optical refocusing in high numerical aperture microscopy |
| work_keys_str_mv | AT botcherbye aberrationfreeopticalrefocusinginhighnumericalaperturemicroscopy AT juskaitisr aberrationfreeopticalrefocusinginhighnumericalaperturemicroscopy AT boothm aberrationfreeopticalrefocusinginhighnumericalaperturemicroscopy AT wilsont aberrationfreeopticalrefocusinginhighnumericalaperturemicroscopy |