Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056.
A significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae genomic island ICEHin1056 is an archetype of a genomic island that accounts for pandemic spread of antibiotics resistance. ICEHin1056 has modular structure and harbors modules involved in type IV...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797062998303440896 |
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| author | Juhas, M Dimopoulou, I Robinson, E Elamin, A Harding, R Hood, D Crook, D |
| author_facet | Juhas, M Dimopoulou, I Robinson, E Elamin, A Harding, R Hood, D Crook, D |
| author_sort | Juhas, M |
| collection | OXFORD |
| description | A significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae genomic island ICEHin1056 is an archetype of a genomic island that accounts for pandemic spread of antibiotics resistance. ICEHin1056 has modular structure and harbors modules involved in type IV secretion and integration. Previous studies have shown that ICEHin1056 encodes a functional type IV secretion system; however, other modules have not been characterized yet. Here we show that the module on the 5' extremity of ICEHin1056 consists of 15 genes that are well conserved in a number of related genomic islands. Furthermore by disrupting six genes of the investigated module of ICEHin1056 by site-specific mutagenesis we demonstrate that in addition to type IV secretion system module, the investigated module is also important for the successful conjugal transfer of ICEHin1056 from donor to recipient cells. |
| first_indexed | 2024-03-06T20:53:33Z |
| format | Journal article |
| id | oxford-uuid:386bb3aa-a2e9-495e-b7e6-ca777cdc6c23 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:53:33Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:386bb3aa-a2e9-495e-b7e6-ca777cdc6c232022-03-26T13:49:53ZIdentification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:386bb3aa-a2e9-495e-b7e6-ca777cdc6c23EnglishSymplectic Elements at Oxford2013Juhas, MDimopoulou, IRobinson, EElamin, AHarding, RHood, DCrook, DA significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae genomic island ICEHin1056 is an archetype of a genomic island that accounts for pandemic spread of antibiotics resistance. ICEHin1056 has modular structure and harbors modules involved in type IV secretion and integration. Previous studies have shown that ICEHin1056 encodes a functional type IV secretion system; however, other modules have not been characterized yet. Here we show that the module on the 5' extremity of ICEHin1056 consists of 15 genes that are well conserved in a number of related genomic islands. Furthermore by disrupting six genes of the investigated module of ICEHin1056 by site-specific mutagenesis we demonstrate that in addition to type IV secretion system module, the investigated module is also important for the successful conjugal transfer of ICEHin1056 from donor to recipient cells. |
| spellingShingle | Juhas, M Dimopoulou, I Robinson, E Elamin, A Harding, R Hood, D Crook, D Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title | Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title_full | Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title_fullStr | Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title_full_unstemmed | Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title_short | Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. |
| title_sort | identification of another module involved in the horizontal transfer of the haemophilus genomic island icehin1056 |
| work_keys_str_mv | AT juhasm identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT dimopouloui identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT robinsone identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT elamina identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT hardingr identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT hoodd identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 AT crookd identificationofanothermoduleinvolvedinthehorizontaltransferofthehaemophilusgenomicislandicehin1056 |