Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion.
Tumor invasion into blood and/or lymphatic channels is an important component of cancer staging and prognosis. Standard pathological methods do not provide sufficient contrast to discriminate between invasion into each type of vessel and are complicated by tissue retraction artifacts. We evaluated t...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2008
|
| _version_ | 1797063031581048832 |
|---|---|
| author | O'Donnell, R Feldman, M Mick, R Muschel, R |
| author_facet | O'Donnell, R Feldman, M Mick, R Muschel, R |
| author_sort | O'Donnell, R |
| collection | OXFORD |
| description | Tumor invasion into blood and/or lymphatic channels is an important component of cancer staging and prognosis. Standard pathological methods do not provide sufficient contrast to discriminate between invasion into each type of vessel and are complicated by tissue retraction artifacts. We evaluated the ability of a triple-stain immunohistochemical method, combining cytokeratin, CD34, and podoplanin stains in a single section, to distinguish blood from lymphatic vascular invasion in oral squamous cell carcinoma and confirmed its results using multispectral analysis. The triple-stain method was significantly more sensitive in detecting invasive events than the standard hematoxylin and eosin staining method and easily discriminated between blood and lymphatic vessel invasion. Invasive events were present in blood and/or lymphatic vessels in the majority of patients with and without presentation of lymph node metastasis, indicating that vessel invasion in this cancer model is common and is not a rate-limiting step for lymph node metastasis. |
| first_indexed | 2024-03-06T20:54:03Z |
| format | Journal article |
| id | oxford-uuid:3891ea5f-ac80-45c4-9f70-affc85688aa2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:54:03Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:3891ea5f-ac80-45c4-9f70-affc85688aa22022-03-26T13:50:47ZImmunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3891ea5f-ac80-45c4-9f70-affc85688aa2EnglishSymplectic Elements at Oxford2008O'Donnell, RFeldman, MMick, RMuschel, RTumor invasion into blood and/or lymphatic channels is an important component of cancer staging and prognosis. Standard pathological methods do not provide sufficient contrast to discriminate between invasion into each type of vessel and are complicated by tissue retraction artifacts. We evaluated the ability of a triple-stain immunohistochemical method, combining cytokeratin, CD34, and podoplanin stains in a single section, to distinguish blood from lymphatic vascular invasion in oral squamous cell carcinoma and confirmed its results using multispectral analysis. The triple-stain method was significantly more sensitive in detecting invasive events than the standard hematoxylin and eosin staining method and easily discriminated between blood and lymphatic vessel invasion. Invasive events were present in blood and/or lymphatic vessels in the majority of patients with and without presentation of lymph node metastasis, indicating that vessel invasion in this cancer model is common and is not a rate-limiting step for lymph node metastasis. |
| spellingShingle | O'Donnell, R Feldman, M Mick, R Muschel, R Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title | Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title_full | Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title_fullStr | Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title_full_unstemmed | Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title_short | Immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion. |
| title_sort | immunohistochemical method identifies lymphovascular invasion in a majority of oral squamous cell carcinomas and discriminates between blood and lymphatic vessel invasion |
| work_keys_str_mv | AT odonnellr immunohistochemicalmethodidentifieslymphovascularinvasioninamajorityoforalsquamouscellcarcinomasanddiscriminatesbetweenbloodandlymphaticvesselinvasion AT feldmanm immunohistochemicalmethodidentifieslymphovascularinvasioninamajorityoforalsquamouscellcarcinomasanddiscriminatesbetweenbloodandlymphaticvesselinvasion AT mickr immunohistochemicalmethodidentifieslymphovascularinvasioninamajorityoforalsquamouscellcarcinomasanddiscriminatesbetweenbloodandlymphaticvesselinvasion AT muschelr immunohistochemicalmethodidentifieslymphovascularinvasioninamajorityoforalsquamouscellcarcinomasanddiscriminatesbetweenbloodandlymphaticvesselinvasion |