Inflammatory stroke extracellular vesicles induce macrophage activation
BACKGROUND AND PURPOSE: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of s...
| Κύριοι συγγραφείς: | , , , , , , , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
American Heart Association
2017
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| _version_ | 1826267444242546688 |
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| author | Couch, Y Akbar, N Davis, S Fischer, R Dickens, A Neuhaus, A Burgess, A Rothwell, P Buchan, A |
| author_facet | Couch, Y Akbar, N Davis, S Fischer, R Dickens, A Neuhaus, A Burgess, A Rothwell, P Buchan, A |
| author_sort | Couch, Y |
| collection | OXFORD |
| description | BACKGROUND AND PURPOSE: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs. METHODS: EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression. RESULTS: EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression. CONCLUSIONS: Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells. |
| first_indexed | 2024-03-06T20:54:18Z |
| format | Journal article |
| id | oxford-uuid:38a76e13-ff49-47dc-a580-3ecbae70da6c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:54:18Z |
| publishDate | 2017 |
| publisher | American Heart Association |
| record_format | dspace |
| spelling | oxford-uuid:38a76e13-ff49-47dc-a580-3ecbae70da6c2022-03-26T13:51:26ZInflammatory stroke extracellular vesicles induce macrophage activationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:38a76e13-ff49-47dc-a580-3ecbae70da6cEnglishSymplectic Elements at OxfordAmerican Heart Association2017Couch, YAkbar, NDavis, SFischer, RDickens, ANeuhaus, ABurgess, ARothwell, PBuchan, ABACKGROUND AND PURPOSE: Extracellular vesicles (EVs) are protein-lipid complexes released from cells, as well as actively exocytosed, as part of normal physiology, but also during pathological processes such as those occurring during a stroke. Our aim was to determine the inflammatory potential of stroke EVs. METHODS: EVs were quantified and analyzed in the sera of patients after an acute stroke (<24 hours; OXVASC [Oxford Vascular Study]). Isolated EV fractions were subjected to untargeted proteomic analysis by liquid chromatography mass-spectrometry/mass-spectrometry and then applied to macrophages in culture to investigate inflammatory gene expression. RESULTS: EV number, but not size, is significantly increased in stroke patients when compared to age-matched controls. Proteomic analysis reveals an overall increase in acute phase proteins, including C-reactive protein. EV fractions applied to monocyte-differentiated macrophage cultures induced inflammatory gene expression. CONCLUSIONS: Together these data show that EVs from stroke patients are proinflammatory in nature and are capable of inducing inflammation in immune cells. |
| spellingShingle | Couch, Y Akbar, N Davis, S Fischer, R Dickens, A Neuhaus, A Burgess, A Rothwell, P Buchan, A Inflammatory stroke extracellular vesicles induce macrophage activation |
| title | Inflammatory stroke extracellular vesicles induce macrophage activation |
| title_full | Inflammatory stroke extracellular vesicles induce macrophage activation |
| title_fullStr | Inflammatory stroke extracellular vesicles induce macrophage activation |
| title_full_unstemmed | Inflammatory stroke extracellular vesicles induce macrophage activation |
| title_short | Inflammatory stroke extracellular vesicles induce macrophage activation |
| title_sort | inflammatory stroke extracellular vesicles induce macrophage activation |
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