Using siRNA to define functional interactions between melanopsin and multiple G Protein partners.
Melanopsin expressing photosensitive retinal ganglion cells (pRGCs) represent a third class of ocular photoreceptors and mediate a range of non-image forming responses to light. Melanopsin is a G protein coupled receptor (GPCR) and existing data suggest that it employs a membrane bound signalling ca...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2015
|
| _version_ | 1797063063478730752 |
|---|---|
| author | Hughes, S Jagannath, A Hickey, D Gatti, S Wood, M Peirson, S Foster, R Hankins, M |
| author_facet | Hughes, S Jagannath, A Hickey, D Gatti, S Wood, M Peirson, S Foster, R Hankins, M |
| author_sort | Hughes, S |
| collection | OXFORD |
| description | Melanopsin expressing photosensitive retinal ganglion cells (pRGCs) represent a third class of ocular photoreceptors and mediate a range of non-image forming responses to light. Melanopsin is a G protein coupled receptor (GPCR) and existing data suggest that it employs a membrane bound signalling cascade involving Gnaq/11 type G proteins. However, to date the precise identity of the Gα subunits involved in melanopsin phototransduction remains poorly defined. Here we show that Gnaq, Gna11 and Gna14 are highly co-expressed in pRGCs of the mouse retina. Furthermore, using RNAi based gene silencing we show that melanopsin can signal via Gnaq, Gna11 or Gna14 in vitro, and demonstrate that multiple members of the Gnaq/11 subfamily, including Gna14 and at least Gnaq or Gna11, can participate in melanopsin phototransduction in vivo and contribute to the pupillary light responses of mice lacking rod and cone photoreceptors. This diversity of G protein interactions suggests additional complexity in the melanopsin phototransduction cascade and may provide a basis for generating the diversity of light responses observed from pRGC subtypes. |
| first_indexed | 2024-03-06T20:54:30Z |
| format | Journal article |
| id | oxford-uuid:38ba60d4-dd93-46b0-8a4e-422350b03a05 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:54:30Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oxford-uuid:38ba60d4-dd93-46b0-8a4e-422350b03a052022-03-26T13:51:43ZUsing siRNA to define functional interactions between melanopsin and multiple G Protein partners.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:38ba60d4-dd93-46b0-8a4e-422350b03a05EnglishSymplectic Elements at Oxford2015Hughes, SJagannath, AHickey, DGatti, SWood, MPeirson, SFoster, RHankins, MMelanopsin expressing photosensitive retinal ganglion cells (pRGCs) represent a third class of ocular photoreceptors and mediate a range of non-image forming responses to light. Melanopsin is a G protein coupled receptor (GPCR) and existing data suggest that it employs a membrane bound signalling cascade involving Gnaq/11 type G proteins. However, to date the precise identity of the Gα subunits involved in melanopsin phototransduction remains poorly defined. Here we show that Gnaq, Gna11 and Gna14 are highly co-expressed in pRGCs of the mouse retina. Furthermore, using RNAi based gene silencing we show that melanopsin can signal via Gnaq, Gna11 or Gna14 in vitro, and demonstrate that multiple members of the Gnaq/11 subfamily, including Gna14 and at least Gnaq or Gna11, can participate in melanopsin phototransduction in vivo and contribute to the pupillary light responses of mice lacking rod and cone photoreceptors. This diversity of G protein interactions suggests additional complexity in the melanopsin phototransduction cascade and may provide a basis for generating the diversity of light responses observed from pRGC subtypes. |
| spellingShingle | Hughes, S Jagannath, A Hickey, D Gatti, S Wood, M Peirson, S Foster, R Hankins, M Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title | Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title_full | Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title_fullStr | Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title_full_unstemmed | Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title_short | Using siRNA to define functional interactions between melanopsin and multiple G Protein partners. |
| title_sort | using sirna to define functional interactions between melanopsin and multiple g protein partners |
| work_keys_str_mv | AT hughess usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT jagannatha usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT hickeyd usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT gattis usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT woodm usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT peirsons usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT fosterr usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners AT hankinsm usingsirnatodefinefunctionalinteractionsbetweenmelanopsinandmultiplegproteinpartners |