Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Es...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2014
|
| _version_ | 1797063063880335360 |
|---|---|
| author | Bird, L Rada, H Flanagan, J Diprose, J Gilbert, R Owens, R |
| author_facet | Bird, L Rada, H Flanagan, J Diprose, J Gilbert, R Owens, R |
| author_sort | Bird, L |
| collection | OXFORD |
| description | In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. |
| first_indexed | 2024-03-06T20:54:30Z |
| format | Journal article |
| id | oxford-uuid:38ba9e72-654d-46cc-9636-a5bc1acf82de |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:54:30Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:38ba9e72-654d-46cc-9636-a5bc1acf82de2022-03-26T13:51:44ZApplication of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:38ba9e72-654d-46cc-9636-a5bc1acf82deEnglishSymplectic Elements at Oxford2014Bird, LRada, HFlanagan, JDiprose, JGilbert, ROwens, RIn-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Escherichia coli of constructs generated by In-Fusion™ is also outlined. |
| spellingShingle | Bird, L Rada, H Flanagan, J Diprose, J Gilbert, R Owens, R Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title | Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title_full | Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title_fullStr | Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title_full_unstemmed | Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title_short | Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors. |
| title_sort | application of in fusion™ cloning for the parallel construction of e coli expression vectors |
| work_keys_str_mv | AT birdl applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors AT radah applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors AT flanaganj applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors AT diprosej applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors AT gilbertr applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors AT owensr applicationofinfusioncloningfortheparallelconstructionofecoliexpressionvectors |