Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

Application of In-Fusion™ cloning for the parallel construction of E. coli expression vectors.

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In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-ranging uses in molecular biology. In this chapter we describe the protocols used in the OPPF-UK to design and construct expression vectors using In-Fusion™. Our method for small scale expression screening in Es...

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Bibliographic Details
Main Authors:	Bird, L, Rada, H, Flanagan, J, Diprose, J, Gilbert, R, Owens, R
Format:	Journal article
Language:	English
Published:	2014

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