Wavelength considerations in confocal microscopy of botanical specimens
We have used a multiple-laser confocal microscope with lines 325, 442, 488, 514 and 633 nm to investigate optical sectioning of botanical specimens over a wide range of wavelengths. The 442-nm line allowed efficient excitation of Chromomycin A3, with minimal background autofluorescence, to visualize...
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| Format: | Journal article |
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Wiley
1992
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