High-throughput production of human proteins for crystallization: the SGC experience.
Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of approximately 40...
Hlavní autoři: | Savitsky, P, Bray, J, Cooper, C, Marsden, B, Mahajan, P, Burgess-Brown, N, Gileadi, O |
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Médium: | Journal article |
Jazyk: | English |
Vydáno: |
Elsevier
2010
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