A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus.
The killifish, Fundulus heteroclitus, is a euryhaline teleost fish capable of adapting rapidly to transfer from freshwater (FW) to four times seawater (SW). To investigate osmoregulation at a molecular level, a 5.7-kilobase cDNA homologous to human cystic fibrosis transmembrane conductance regulator...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1998
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| _version_ | 1797063207844577280 |
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| author | Singer, T Tucker, S Marshall, W Higgins, C |
| author_facet | Singer, T Tucker, S Marshall, W Higgins, C |
| author_sort | Singer, T |
| collection | OXFORD |
| description | The killifish, Fundulus heteroclitus, is a euryhaline teleost fish capable of adapting rapidly to transfer from freshwater (FW) to four times seawater (SW). To investigate osmoregulation at a molecular level, a 5.7-kilobase cDNA homologous to human cystic fibrosis transmembrane conductance regulator (hCFTR) was isolated from a gill cDNA library from SW-adapted killifish. This cDNA encodes a protein product (kfCFTR) that is 59% identical to hCFTR, the most divergent form of CFTR characterized to date. Expression of kfCFTR in Xenopus oocytes generated adenosine 3',5'-cyclic monophosphate-activated, Cl(-)-selective currents similar to those generated by hCFTR. In SW-adapted killifish, kfCFTR was expressed at high levels in the gill, opercular epithelium, and intestine. After abrupt exposure of FW-adapted killifish to SW, kfCFTR expression in the gill increased severalfold, suggesting a role for kfCFTR in salinity adaptation. Under similar conditions, plasma Na+ levels rose significantly after 8 h and then fell, although it is not known whether these changes are directly responsible for the changes in kfCFTR expression. The killifish provides a unique opportunity to understand teleost osmoregulation and the role of CFTR. |
| first_indexed | 2024-03-06T20:56:31Z |
| format | Journal article |
| id | oxford-uuid:39737644-c7dd-41bc-bb0d-c3ce3adc094e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:56:31Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:39737644-c7dd-41bc-bb0d-c3ce3adc094e2022-03-26T13:55:32ZA divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:39737644-c7dd-41bc-bb0d-c3ce3adc094eEnglishSymplectic Elements at Oxford1998Singer, TTucker, SMarshall, WHiggins, CThe killifish, Fundulus heteroclitus, is a euryhaline teleost fish capable of adapting rapidly to transfer from freshwater (FW) to four times seawater (SW). To investigate osmoregulation at a molecular level, a 5.7-kilobase cDNA homologous to human cystic fibrosis transmembrane conductance regulator (hCFTR) was isolated from a gill cDNA library from SW-adapted killifish. This cDNA encodes a protein product (kfCFTR) that is 59% identical to hCFTR, the most divergent form of CFTR characterized to date. Expression of kfCFTR in Xenopus oocytes generated adenosine 3',5'-cyclic monophosphate-activated, Cl(-)-selective currents similar to those generated by hCFTR. In SW-adapted killifish, kfCFTR was expressed at high levels in the gill, opercular epithelium, and intestine. After abrupt exposure of FW-adapted killifish to SW, kfCFTR expression in the gill increased severalfold, suggesting a role for kfCFTR in salinity adaptation. Under similar conditions, plasma Na+ levels rose significantly after 8 h and then fell, although it is not known whether these changes are directly responsible for the changes in kfCFTR expression. The killifish provides a unique opportunity to understand teleost osmoregulation and the role of CFTR. |
| spellingShingle | Singer, T Tucker, S Marshall, W Higgins, C A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title | A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title_full | A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title_fullStr | A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title_full_unstemmed | A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title_short | A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus. |
| title_sort | divergent cftr homologue highly regulated salt transport in the euryhaline teleost f heteroclitus |
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