pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.

The physiological role of the inwardly rectifying potassium channel, Kir5.1, is poorly understood, as is the molecular identity of many renal potassium channels. In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir...

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Main Authors: Tucker, S, Imbrici, P, Salvatore, L, D'Adamo, M, Pessia, M
Format: Journal article
Language:English
Published: 2000
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author Tucker, S
Imbrici, P
Salvatore, L
D'Adamo, M
Pessia, M
author_facet Tucker, S
Imbrici, P
Salvatore, L
D'Adamo, M
Pessia, M
author_sort Tucker, S
collection OXFORD
description The physiological role of the inwardly rectifying potassium channel, Kir5.1, is poorly understood, as is the molecular identity of many renal potassium channels. In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir4.1 is also expressed. Moreover, we also show that Kir5.1/Kir4.1 heteromeric channel activity is extremely sensitive to inhibition by intracellular acidification and that this novel property is conferred predominantly by the Kir5.1 subunit. These findings suggest that Kir5.1/Kir4.1 heteromeric channels are likely to exist in vivo and implicate an important and novel functional role for the Kir5.1 subunit.
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spelling oxford-uuid:3a07c5bb-7117-4388-bf2a-714854e41ee42022-03-26T13:59:07ZpH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3a07c5bb-7117-4388-bf2a-714854e41ee4EnglishSymplectic Elements at Oxford2000Tucker, SImbrici, PSalvatore, LD'Adamo, MPessia, MThe physiological role of the inwardly rectifying potassium channel, Kir5.1, is poorly understood, as is the molecular identity of many renal potassium channels. In this study we have used Kir5.1-specific antibodies to reveal abundant expression of Kir5.1 in renal tubular epithelial cells, where Kir4.1 is also expressed. Moreover, we also show that Kir5.1/Kir4.1 heteromeric channel activity is extremely sensitive to inhibition by intracellular acidification and that this novel property is conferred predominantly by the Kir5.1 subunit. These findings suggest that Kir5.1/Kir4.1 heteromeric channels are likely to exist in vivo and implicate an important and novel functional role for the Kir5.1 subunit.
spellingShingle Tucker, S
Imbrici, P
Salvatore, L
D'Adamo, M
Pessia, M
pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title_full pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title_fullStr pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title_full_unstemmed pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title_short pH dependence of the inwardly rectifying potassium channel, Kir5.1, and localization in renal tubular epithelia.
title_sort ph dependence of the inwardly rectifying potassium channel kir5 1 and localization in renal tubular epithelia
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AT salvatorel phdependenceoftheinwardlyrectifyingpotassiumchannelkir51andlocalizationinrenaltubularepithelia
AT dadamom phdependenceoftheinwardlyrectifyingpotassiumchannelkir51andlocalizationinrenaltubularepithelia
AT pessiam phdependenceoftheinwardlyrectifyingpotassiumchannelkir51andlocalizationinrenaltubularepithelia