MEASUREMENTS OF CYTOPLASMIC PH IN INSULIN-RELEASING CELLS WITH INTRACELLULARLY TRAPPED PHENOL RED

MEASUREMENTS OF CYTOPLASMIC PH IN INSULIN-RELEASING CELLS WITH INTRACELLULARLY TRAPPED PHENOL RED

Cytoplasmic pH was measured in insulin-releasing clonal RINm5F cells using a new technique. The cells were permeabilized by electric discharges and loaded with phenol red. After resealing, changes in the absorbance of the intracellular indicator was monitored by dual wavelength spectrophotometry. Th...

Full description

Bibliographic Details
Main Authors:	Gylfe, E, Berggren, P, Rorsman, P
Format:	Journal article
Language:	English
Published:	1986

Similar Items

Reduction of the cytosolic calcium activity in clonal insulin-releasing cells exposed to glucose.
by: Rorsman, P, et al.
Published: (1983)

CYTOPLASMIC PH OF MOUSE PANCREATIC BETA-CELLS AS MEASURED BY THE FLUORESCENT PH-INDICATOR QUENE-1
by: Arkhammar, P, et al.
Published: (1986)

Intracellular pH and the stimulus-secretion coupling in insulin-producing RINm5F cells.
by: Juntti-Berggren, L, et al.
Published: (1992)

D-GLUCOSE AND D-GLYCERALDEHYDE HAVE OPPOSITE EFFECTS ON CYTOPLASMIC PH OF MOUSE PANCREATIC B-CELLS WHEN MEASURED BY THE FLUORESCENT PH-INDICATOR QUENE-1
by: Arkhammar, P, et al.
Published: (1986)

Measurements of membrane potential, transmembrane 45Ca fluxes, cytoplasmic free Ca2+ concentration and insulin release by transplantable rat insulinoma cells maintained in tissue culture.
by: Flatt, P, et al.
Published: (1988)

Suppression of insulin release by galanin and somatostatin is mediated by a G-protein. An effect involving repolarization and reduction in cytoplasmic free Ca2+ concentration.
by: Nilsson, T, et al.
Published: (1989)

Direct evidence for opposite effects of D-glucose and D-glyceraldehyde on cytoplasmic pH of mouse pancreatic beta-cells.
by: Arkhammar, P, et al.
Published: (1986)

Inhibition of glucose-stimulated insulin release by alpha 2-adrenoceptor activation is parallelled by both a repolarization and a reduction in cytoplasmic free Ca2+ concentration.
by: Nilsson, T, et al.
Published: (1988)

MEMBRANE-POTENTIAL, TRANSMEMBRANE CA-45 FLUXES, CYTOPLASMIC FREE CA-2+ CONCENTRATION ([CA-2+]I) AND INSULIN RELEASE BY TRANSPLANTABLE RAT INSULINOMA CELLS
by: Flatt, P, et al.
Published: (1988)

Ca(2+)-induced Ca2+ release in insulin-secreting cells.
by: Islam, MS, et al.
Published: (1992)

Glucose-induced increase in cytoplasmic pH in pancreatic beta-cells is mediated by Na+/H+ exchange, an effect not dependent on protein kinase C.
by: Juntti-Berggren, L, et al.
Published: (1991)

REGULATION OF INSULIN RELEASE - STUDIES EMPLOYING THE PATCH-CLAMP TECHNIQUE AND FLUORESCENT-PROBES
by: Berggren, P, et al.
Published: (1991)

Ca2+ transport in pancreatic beta-cells during glucose stimulation of insulin secretion.
by: Hellman, B, et al.
Published: (1980)

Direct measurements of increased free cytoplasmic Ca2+ in mouse pancreatic beta-cells following stimulation by hypoglycemic sulfonylureas.
by: Abrahamsson, H, et al.
Published: (1985)

Glucagon-like peptide I increases cytoplasmic calcium in insulin-secreting beta TC3-cells by enhancement of intracellular calcium mobilization.
by: Gromada, J, et al.
Published: (1995)

Depolarization-independent net uptake of calcium into clonal insulin-releasing cells exposed to glucose.
by: Gylfe, E, et al.
Published: (1983)

CA2+ MOVEMENTS DURING STIMULATION OF INSULIN RELEASE BY REMOVAL OF EXTRACELLULAR NA+
by: Rorsman, P, et al.
Published: (1980)

CLONIDINE INHIBITION OF GLUCOSE-STIMULATED INSULIN-RELEASE INVOLVES REDUCTION OF THE CA-2+ CURRENT
by: Rorsman, P, et al.
Published: (1987)

Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells.
by: Kindmark, H, et al.
Published: (1991)

SERUM FROM INSULIN-DEPENDENT DIABETICS INCREASE L-TYPE CA-2+-CHANNEL ACTIVITY AND CYTOPLASMIC FREE CA-2+ IN INSULIN-PRODUCING CELLS
by: Junttiberggren, L, et al.
Published: (1992)

Diabetes - when insulin is trapped in the secretory granule?
by: Rorsman, P
Published: (2008)

Extracellular ATP increases cytoplasmic free Ca2+ concentration in clonal insulin-producing RINm5F cells. A mechanism involving direct interaction with both release and refilling of the inositol 1,4,5-trisphosphate-sensitive Ca2+ pool.
by: Arkhammar, P, et al.
Published: (1990)

The dual action of glucose on the cytosolic Ca2+ activity in pancreatic beta-cells. Demonstration of an inhibitory effect of glucose on insulin release in the mouse and man.
by: Hellman, B, et al.
Published: (1985)

Inhibition of ATP-regulated K+ channels precedes depolarization-induced increase in cytoplasmic free Ca2+ concentration in pancreatic beta-cells.
by: Arkhammar, P, et al.
Published: (1987)

Intracellular pH Regulation of Skeletal Muscle in the Milieu of Insulin Signaling
by: Dheeraj Kumar Posa, et al.
Published: (2020-09-01)

Regulation of glucagon release from pancreatic A-cells.
by: Rorsman, P, et al.
Published: (1991)

Palmitate potentiates glucose-induced insulin secretion by increasing cytoplasmic Ca2+and electrical activity in mouse B-cells
by: Olofsson, C, et al.
Published: (2001)

Cytoplasmic calcium transients due to single action potentials and voltage-clamp depolarizations in mouse pancreatic B-cells.
by: Rorsman, P, et al.
Published: (1992)

Glucose in glucagon release.
by: Rorsman, P, et al.
Published: (1990)

BICARBONATE AND CYTOPLASMIC PH
by: Buckler, K, et al.
Published: (1990)

Intracellular pH, cytosolic calcium concentration and electrical activity in RINm5F insulinoma cells.
by: Best, L, et al.
Published: (1994)

Protein kinase C activity affects glucose-induced oscillations in cytoplasmic free Ca2+ in the pancreatic B-cell.
by: Kindmark, H, et al.
Published: (1992)

Voltage-activated Na+ currents and their suppression by phorbol ester in clonal insulin-producing RINm5F cells.
by: Rorsman, P, et al.
Published: (1986)

Calcium-independent potentiation of insulin release by cyclic AMP in single beta-cells.
by: Ammälä, C, et al.
Published: (1993)

Intracellular antigens associated with the cytoplasmic surface of phagolysosomes.
by: Vaux, D, et al.
Published: (1985)

Does galanin inhibit insulin secretion by opening of the ATP-regulated K+ channel in the beta-cell?
by: Ahrén, B, et al.
Published: (1989)

Interaction with the inositol 1,4,5-trisphosphate receptor promotes Ca2+ sequestration in permeabilised insulin-secreting cells.
by: Islam, MS, et al.
Published: (1991)

EXTRACELLULAR PH TRANSIENTS MEASURED WITH PHENOL RED AND PH-SELECTIVE MICROELECTRODES IN ISOLATED SHEEP CARDIAC PURKINJE-FIBERS
by: Bountra, C, et al.
Published: (1988)

The bending rigidity of the red blood cell cytoplasmic membrane
by: Sebastian Himbert, et al.
Published: (2022-01-01)

Quantal ATP release in rat beta-cells by exocytosis of insulin-containing LDCVs.
by: Karanauskaite, J, et al.
Published: (2009)