2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2
Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Published: |
Royal Society of Chemistry
2018
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| _version_ | 1797063491101655040 |
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| author | Abboud, M McAllister, T Leung, I Chowdhury, R Jorgensen, C Domene, C Mecinović, J Lippl,, K Hancock, R Hopkinson, R Kawamura, A Claridge, T Schofield, C |
| author_facet | Abboud, M McAllister, T Leung, I Chowdhury, R Jorgensen, C Domene, C Mecinović, J Lippl,, K Hancock, R Hopkinson, R Kawamura, A Claridge, T Schofield, C |
| author_sort | Abboud, M |
| collection | OXFORD |
| description | Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding |
| first_indexed | 2024-03-06T21:00:38Z |
| format | Journal article |
| id | oxford-uuid:3ac24af1-3ca0-4ba4-9c27-f26f64a0b7eb |
| institution | University of Oxford |
| last_indexed | 2024-03-06T21:00:38Z |
| publishDate | 2018 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:3ac24af1-3ca0-4ba4-9c27-f26f64a0b7eb2022-03-26T14:03:32Z2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3ac24af1-3ca0-4ba4-9c27-f26f64a0b7ebSymplectic Elements at OxfordRoyal Society of Chemistry2018Abboud, MMcAllister, TLeung, IChowdhury, RJorgensen, CDomene, CMecinović, JLippl,, KHancock, RHopkinson, RKawamura, AClaridge, TSchofield, CProlyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ~50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding |
| spellingShingle | Abboud, M McAllister, T Leung, I Chowdhury, R Jorgensen, C Domene, C Mecinović, J Lippl,, K Hancock, R Hopkinson, R Kawamura, A Claridge, T Schofield, C 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title | 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title_full | 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title_fullStr | 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title_full_unstemmed | 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title_short | 2-oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2 |
| title_sort | 2 oxoglutarate regulates binding of hydroxylated hypoxia inducible factor to prolyl hydroxylase domain 2 |
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