The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand
Dengue represents a major public health problem in Thailand, with all four viral serotypes co-circulating. Dengue virus serotype 4 (DENV-4) is the least frequently sampled serotype, although one that is often associated with hemorrhagic fever during secondary infection. To determine the evolutionary...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Elsevier
2004
|
| Subjects: |
| _version_ | 1797063597628588032 |
|---|---|
| author | Klungthong, C Zhang, C Mammen, M Ubol, S Holmes, E |
| author_facet | Klungthong, C Zhang, C Mammen, M Ubol, S Holmes, E |
| author_sort | Klungthong, C |
| collection | OXFORD |
| description | Dengue represents a major public health problem in Thailand, with all four viral serotypes co-circulating. Dengue virus serotype 4 (DENV-4) is the least frequently sampled serotype, although one that is often associated with hemorrhagic fever during secondary infection. To determine the evolutionary forces shaping the genetic diversity of DENV-4, and particularly whether its changing prevalence could be attributed to instances of adaptive evolution in the viral genome, we undertook a large-scale molecular epidemiological analysis of DENV-4 in Bangkok, Thailand, using both E gene and complete coding region sequences. This analysis revealed extensive genetic diversity within a single locality at a single time, including the discovery of a new and divergent genotype of DENV-4, as well as a pattern of continual lineage turnover. We also recorded the highest average rate of evolutionary change for this serotype, at 1.072 × 10<sup>-3</sup> nucleotide substitutions per site, per year. However, despite this abundant genetic variation, there was no evidence for adaptive evolution in any gene, codon, or lineage of DENV-4, with the highest rate of nonsynonymous substitution observed in NS2A. Consequently, the rapid turnover of DENV-4 lineages through time is most likely the consequence of a high rate of deleterious mutation in the viral genome coupled to seasonal fluctuations in the size of the vector population. |
| first_indexed | 2024-03-06T21:02:11Z |
| format | Journal article |
| id | oxford-uuid:3b3f4aae-7d09-443b-973e-9402dbb286e2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:02:11Z |
| publishDate | 2004 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:3b3f4aae-7d09-443b-973e-9402dbb286e22022-03-26T14:06:30ZThe molecular epidemiology of dengue virus serotype 4 in Bangkok, ThailandJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3b3f4aae-7d09-443b-973e-9402dbb286e2VirusesGenetics (medical sciences)Infectious diseasesEnglishOxford University Research Archive - ValetElsevier2004Klungthong, CZhang, CMammen, MUbol, SHolmes, EDengue represents a major public health problem in Thailand, with all four viral serotypes co-circulating. Dengue virus serotype 4 (DENV-4) is the least frequently sampled serotype, although one that is often associated with hemorrhagic fever during secondary infection. To determine the evolutionary forces shaping the genetic diversity of DENV-4, and particularly whether its changing prevalence could be attributed to instances of adaptive evolution in the viral genome, we undertook a large-scale molecular epidemiological analysis of DENV-4 in Bangkok, Thailand, using both E gene and complete coding region sequences. This analysis revealed extensive genetic diversity within a single locality at a single time, including the discovery of a new and divergent genotype of DENV-4, as well as a pattern of continual lineage turnover. We also recorded the highest average rate of evolutionary change for this serotype, at 1.072 × 10<sup>-3</sup> nucleotide substitutions per site, per year. However, despite this abundant genetic variation, there was no evidence for adaptive evolution in any gene, codon, or lineage of DENV-4, with the highest rate of nonsynonymous substitution observed in NS2A. Consequently, the rapid turnover of DENV-4 lineages through time is most likely the consequence of a high rate of deleterious mutation in the viral genome coupled to seasonal fluctuations in the size of the vector population. |
| spellingShingle | Viruses Genetics (medical sciences) Infectious diseases Klungthong, C Zhang, C Mammen, M Ubol, S Holmes, E The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title | The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title_full | The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title_fullStr | The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title_full_unstemmed | The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title_short | The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand |
| title_sort | molecular epidemiology of dengue virus serotype 4 in bangkok thailand |
| topic | Viruses Genetics (medical sciences) Infectious diseases |
| work_keys_str_mv | AT klungthongc themolecularepidemiologyofdenguevirusserotype4inbangkokthailand AT zhangc themolecularepidemiologyofdenguevirusserotype4inbangkokthailand AT mammenm themolecularepidemiologyofdenguevirusserotype4inbangkokthailand AT ubols themolecularepidemiologyofdenguevirusserotype4inbangkokthailand AT holmese themolecularepidemiologyofdenguevirusserotype4inbangkokthailand AT klungthongc molecularepidemiologyofdenguevirusserotype4inbangkokthailand AT zhangc molecularepidemiologyofdenguevirusserotype4inbangkokthailand AT mammenm molecularepidemiologyofdenguevirusserotype4inbangkokthailand AT ubols molecularepidemiologyofdenguevirusserotype4inbangkokthailand AT holmese molecularepidemiologyofdenguevirusserotype4inbangkokthailand |