Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA
Zika virus (ZIKV) is an emerging mosquito-borne flavivirus, which has recently caused global epidemics with its association with congenital Zika syndrome such as severe microcephaly. The recombinant ZIKV envelope (Env) glycoprotein is useful for immunological applications such as serodiagnosis of ZI...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Humana Press
2020
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| _version_ | 1797063629172899840 |
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| author | Kim, YC Dumoux, M Owens, RJ Reyes-Sandoval, A |
| author_facet | Kim, YC Dumoux, M Owens, RJ Reyes-Sandoval, A |
| author_sort | Kim, YC |
| collection | OXFORD |
| description | Zika virus (ZIKV) is an emerging mosquito-borne flavivirus, which has recently caused global epidemics with its association with congenital Zika syndrome such as severe microcephaly. The recombinant ZIKV envelope (Env) glycoprotein is useful for immunological applications such as serodiagnosis of ZIKV infection and for monitoring immune responses in preclinical and clinical ZIKV vaccine developments. In this chapter, we describe the optimization of production of Zika virus envelope glycoprotein in Human Embryonic Kidney (HEK 293T) cells by small-scale expression followed by large-scale protein production. Small-scale expression of HEK 293T cells allows screening of a large number of vectors simultaneously to select the vectors with best secretory profiles for scale-up in Expi293 mammalian system to maximize the protein yield followed by purification for research and clinical applications. |
| first_indexed | 2024-03-06T21:02:39Z |
| format | Journal article |
| id | oxford-uuid:3b6415dc-aa41-4aac-bf25-828773dd6d4c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:02:39Z |
| publishDate | 2020 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:3b6415dc-aa41-4aac-bf25-828773dd6d4c2022-03-26T14:07:21ZOptimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISAJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3b6415dc-aa41-4aac-bf25-828773dd6d4cEnglishSymplectic ElementsHumana Press2020Kim, YCDumoux, MOwens, RJReyes-Sandoval, AZika virus (ZIKV) is an emerging mosquito-borne flavivirus, which has recently caused global epidemics with its association with congenital Zika syndrome such as severe microcephaly. The recombinant ZIKV envelope (Env) glycoprotein is useful for immunological applications such as serodiagnosis of ZIKV infection and for monitoring immune responses in preclinical and clinical ZIKV vaccine developments. In this chapter, we describe the optimization of production of Zika virus envelope glycoprotein in Human Embryonic Kidney (HEK 293T) cells by small-scale expression followed by large-scale protein production. Small-scale expression of HEK 293T cells allows screening of a large number of vectors simultaneously to select the vectors with best secretory profiles for scale-up in Expi293 mammalian system to maximize the protein yield followed by purification for research and clinical applications. |
| spellingShingle | Kim, YC Dumoux, M Owens, RJ Reyes-Sandoval, A Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title | Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title_full | Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title_fullStr | Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title_full_unstemmed | Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title_short | Optimization of small-scale production of Zika virus envelope glycoprotein by transient expression in HEK293 cells for ELISA |
| title_sort | optimization of small scale production of zika virus envelope glycoprotein by transient expression in hek293 cells for elisa |
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