A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation.
The mammalian nuclear lamina protein lamin B1 is posttranslationally modified by farnesylation, endoproteolysis, and carboxymethylation at a carboxyl-terminal CAAX motif. In this work, we demonstrate that the CAAX endoprotease Rce1 is required for lamin B1 endoproteolysis, demonstrate an independent...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797063635745374208 |
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| author | Maske, C Hollinshead, MS Higbee, N Bergo, M Young, S Vaux, D |
| author_facet | Maske, C Hollinshead, MS Higbee, N Bergo, M Young, S Vaux, D |
| author_sort | Maske, C |
| collection | OXFORD |
| description | The mammalian nuclear lamina protein lamin B1 is posttranslationally modified by farnesylation, endoproteolysis, and carboxymethylation at a carboxyl-terminal CAAX motif. In this work, we demonstrate that the CAAX endoprotease Rce1 is required for lamin B1 endoproteolysis, demonstrate an independent pool of proteolyzed but nonmethylated lamin B1, as well as fully processed lamin B1, in interphase nuclei, and show a role for methylation in the organization of lamin B1 into domains of the nuclear lamina. Deficiency in the endoproteolysis or methylation of lamin B1 results in loss of integrity and deformity of the nuclear lamina. These data show that the organization of the nuclear envelope and lamina is dependent on a mechanism involving the methylation of lamin B1, and they identify a potential mechanism of laminopathy involving a B-type lamin. |
| first_indexed | 2024-03-06T21:02:43Z |
| format | Journal article |
| id | oxford-uuid:3b68a943-fbf6-4d66-a29c-750b172d65c0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:02:43Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:3b68a943-fbf6-4d66-a29c-750b172d65c02022-03-26T14:07:34ZA carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3b68a943-fbf6-4d66-a29c-750b172d65c0EnglishSymplectic Elements at Oxford2003Maske, CHollinshead, MSHigbee, NBergo, MYoung, SVaux, DThe mammalian nuclear lamina protein lamin B1 is posttranslationally modified by farnesylation, endoproteolysis, and carboxymethylation at a carboxyl-terminal CAAX motif. In this work, we demonstrate that the CAAX endoprotease Rce1 is required for lamin B1 endoproteolysis, demonstrate an independent pool of proteolyzed but nonmethylated lamin B1, as well as fully processed lamin B1, in interphase nuclei, and show a role for methylation in the organization of lamin B1 into domains of the nuclear lamina. Deficiency in the endoproteolysis or methylation of lamin B1 results in loss of integrity and deformity of the nuclear lamina. These data show that the organization of the nuclear envelope and lamina is dependent on a mechanism involving the methylation of lamin B1, and they identify a potential mechanism of laminopathy involving a B-type lamin. |
| spellingShingle | Maske, C Hollinshead, MS Higbee, N Bergo, M Young, S Vaux, D A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title | A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title_full | A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title_fullStr | A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title_full_unstemmed | A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title_short | A carboxyl-terminal interaction of lamin B1 is dependent on the CAAX endoprotease Rce1 and carboxymethylation. |
| title_sort | carboxyl terminal interaction of lamin b1 is dependent on the caax endoprotease rce1 and carboxymethylation |
| work_keys_str_mv | AT maskec acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT hollinsheadms acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT higbeen acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT bergom acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT youngs acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT vauxd acarboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT maskec carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT hollinsheadms carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT higbeen carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT bergom carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT youngs carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation AT vauxd carboxylterminalinteractionoflaminb1isdependentonthecaaxendoproteaserce1andcarboxymethylation |