Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

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We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...

书目详细资料
Main Authors:	Hua, H, Kearsey, S
格式:	Journal article
语言:	English
出版:	Oxford University Press 2011
主题:	Zoological sciences

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