Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...

पूर्ण विवरण

ग्रंथसूची विवरण
मुख्य लेखकों: Hua, H, Kearsey, S
स्वरूप: Journal article
भाषा:English
प्रकाशित: Oxford University Press 2011
विषय: