Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...

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Bibliografische gegevens
Hoofdauteurs: Hua, H, Kearsey, S
Formaat: Journal article
Taal:English
Gepubliceerd in: Oxford University Press 2011
Onderwerpen: