Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine
We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromode-oxyuridine. Low concentrations of EdU (1μM) are sufficient to allow detection of incorporation in cells exp...
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| Format: | Journal article |
| Langue: | English |
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Oxford University Press
2011
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