A radical intermediate in tyrosine scission to the CO and CN⁻ ligands of FeFe hydrogenase

A radical intermediate in tyrosine scission to the CO and CN⁻ ligands of FeFe hydrogenase

The radical <em>S</em>-adenosylmethionine (SAM) enzyme HydG lyses free L-tyrosine to produce CO and CN<em><sup>−</sup></em> for the assembly of the catalytic H cluster of FeFe hydrogenase. We used electron paramagnetic resonance spectroscopy to detect and characte...

Bibliográfalaš dieđut
Váldodahkkit:	Kuchenreuther, J, Myers, W, Stich, T, George, S, NejatyJahromy, Y, Swartz, JR, Britt, R
Materiálatiipa:	Journal article
Giella:	English
Almmustuhtton:	American Association for the Advancement of Science 2013

Geahča maid

The HydG enzyme generates an Fe(CO)2(CN) synthon in assembly of the FeFe hydrogenase H-cluster.
Dahkki: Kuchenreuther, J, et al.
Almmustuhtton: (2014)

The cyanide ligands of [FeFe] hydrogenase: pulse EPR studies of (13)C and (15)N-labeled H-cluster.
Dahkki: Myers, W, et al.
Almmustuhtton: (2014)

Cell-free H-cluster synthesis and [FeFe] hydrogenase activation: all five CO and CN⁻ ligands derive from tyrosine.
Dahkki: Jon M Kuchenreuther, et al.
Almmustuhtton: (2011-01-01)

Tyrosine, cysteine, and S-adenosyl methionine stimulate in vitro [FeFe] hydrogenase activation.
Dahkki: Jon M Kuchenreuther, et al.
Almmustuhtton: (2009-10-01)

New insights into [FeFe] hydrogenase activation and maturase function.
Dahkki: Jon M Kuchenreuther, et al.
Almmustuhtton: (2012-01-01)

High-yield expression of heterologous [FeFe] hydrogenases in Escherichia coli.
Dahkki: Jon M Kuchenreuther, et al.
Almmustuhtton: (2010-01-01)

Nuclear resonance vibrational spectroscopy and electron paramagnetic resonance spectroscopy of 57Fe-enriched [FeFe] hydrogenase indicate stepwise assembly of the H-cluster.
Dahkki: Kuchenreuther, J, et al.
Almmustuhtton: (2013)

Reactions of [FeFe]-hydrogenase with carbon monoxide and formaldehyde
Dahkki: Foster, C, et al.
Almmustuhtton: (2012)

Fantastic [FeFe]-Hydrogenases and Where to Find Them
Dahkki: Simone Morra
Almmustuhtton: (2022-03-01)

A cell-free microtiter plate screen for improved [FeFe] hydrogenases.
Dahkki: James A Stapleton, et al.
Almmustuhtton: (2010-05-01)

The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases
Dahkki: Nathaniel A. Losey, et al.
Almmustuhtton: (2020-06-01)

Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.
Dahkki: James A Stapleton, et al.
Almmustuhtton: (2010-01-01)

Importance of the protein framework for catalytic activity of [FeFe]-hydrogenases.
Dahkki: Knörzer, P, et al.
Almmustuhtton: (2012)

Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli
Dahkki: Ciarán L. Kelly, et al.
Almmustuhtton: (2015-12-01)

Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase
Dahkki: Chris Furlan, et al.
Almmustuhtton: (2022-08-01)

Insights into Triazolylidene Ligands Behaviour at a Di-Iron Site Related to [FeFe]-Hydrogenases
Dahkki: Andrea Mele, et al.
Almmustuhtton: (2022-07-01)

How oxygen attacks [FeFe] hydrogenases from photosynthetic organisms.
Dahkki: Stripp, S, et al.
Almmustuhtton: (2009)

Mono- and Dinuclear Carbonyl Dithiolene Complexes Related to the [FeFe]-Hydrogenases
Dahkki: Mohamed Kdider, et al.
Almmustuhtton: (2023-09-01)

The [FeFe] hydrogenase of <it>Nyctotherus ovalis </it>has a chimeric origin
Dahkki: Jouany Jean-Pierre, et al.
Almmustuhtton: (2007-11-01)

Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases
Dahkki: Jifu Duan, et al.
Almmustuhtton: (2018-11-01)

Unraveling the H-cluster biosynthetic pathway: Investigations of radical SAM chemistry catalyzed by the HydG maturase
Dahkki: Kuchenreuther, J, et al.
Almmustuhtton: (2013)

Wiring an [FeFe]-hydrogenase with photosystem I for light-induced hydrogen production.
Dahkki: Lubner, C, et al.
Almmustuhtton: (2010)

Inhibition of [FeFe]-hydrogenases by formaldehyde and wider mechanistic implications for biohydrogen activation.
Dahkki: Foster, C, et al.
Almmustuhtton: (2012)

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase
Dahkki: Yacoby, Iftach, et al.
Almmustuhtton: (2012)

Formaldehyde--a rapid and reversible inhibitor of hydrogen production by [FeFe]-hydrogenases.
Dahkki: Wait, A, et al.
Almmustuhtton: (2011)

Frequency and potential dependence of reversible electrocatalytic hydrogen interconversion by [FeFe]-hydrogenases
Dahkki: Pandey, K, et al.
Almmustuhtton: (2019)

Aza-Diphosphido-Bridged Di-Iron Complexes Related to the [FeFe]-Hydrogenases
Dahkki: Pankaj Das, et al.
Almmustuhtton: (2024-03-01)

The Contribution of Proton-Donor pKa on Reactivity Profiles of [FeFe]-hydrogenases
Dahkki: Effie C. Kisgeropoulos, et al.
Almmustuhtton: (2022-09-01)

Optimized expression and purification for high-activity preparations of algal [FeFe]-hydrogenase.
Dahkki: Iftach Yacoby, et al.
Almmustuhtton: (2012-01-01)

Spectroscopical Investigations on the Redox Chemistry of [FeFe]-Hydrogenases in the Presence of Carbon Monoxide
Dahkki: Konstantin Laun, et al.
Almmustuhtton: (2018-07-01)

Electrochemical Investigations of the Mechanism of Assembly of the Active-Site H-Cluster of [FeFe]-Hydrogenases
Dahkki: Megarity, C, et al.
Almmustuhtton: (2016)

Complex Multimeric [FeFe] Hydrogenases: Biochemistry, Physiology and New Opportunities for the Hydrogen Economy
Dahkki: Kai Schuchmann, et al.
Almmustuhtton: (2018-12-01)

Proposed Mechanism for the Biosynthesis of the [FeFe] Hydrogenase H‑Cluster: Central Roles for the Radical SAM Enzymes HydG and HydE
Dahkki: R. David Britt, et al.
Almmustuhtton: (2021-10-01)

Quantum Chemical Topological Analysis of [2Fe2S] Core in Novel [FeFe]-Hydrogenase Mimics
Dahkki: Piotr Matczak
Almmustuhtton: (2025-01-01)

Diverse non-canonical electron bifurcating [FeFe]-hydrogenases of separate evolutionary origins in Hydrogenedentota
Dahkki: Xiaowei Zheng, et al.
Almmustuhtton: (2024-09-01)

A synthetic system links FeFe-hydrogenases to essential <it>E. coli </it>sulfur metabolism
Dahkki: Grandl Gerald, et al.
Almmustuhtton: (2011-05-01)

Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
Dahkki: Yacoby, Iftach, et al.
Almmustuhtton: (2012)

Electrochemical investigations of the interconversions between catalytic and inhibited states of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans.
Dahkki: Parkin, A, et al.
Almmustuhtton: (2006)

Triiron Complexes Featuring Azadiphosphine Related to the Active Site of [FeFe]-Hydrogenases: Their Redox Behavior and Protonation
Dahkki: Ahmad Hobballah, et al.
Almmustuhtton: (2024-07-01)

Electrochemical Characterization of a Complex FeFe Hydrogenase, the Electron-Bifurcating Hnd From Desulfovibrio fructosovorans
Dahkki: Aurore Jacq-Bailly, et al.
Almmustuhtton: (2021-01-01)