High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples.
The use of imino sugars for the potential treatment of lysosomal glycolipid storage diseases and hepatitis virus infections requires accurate, quantitative measurement of these compounds in biological samples. We demonstrate here the versatility of cation-exchange chromatography and pulsed amperomet...
| Κύριοι συγγραφείς: | , , , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
2000
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| _version_ | 1826268170316414976 |
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| author | Mellor, H Adam, A Platt, F Dwek, R Butters, T |
| author_facet | Mellor, H Adam, A Platt, F Dwek, R Butters, T |
| author_sort | Mellor, H |
| collection | OXFORD |
| description | The use of imino sugars for the potential treatment of lysosomal glycolipid storage diseases and hepatitis virus infections requires accurate, quantitative measurement of these compounds in biological samples. We demonstrate here the versatility of cation-exchange chromatography and pulsed amperometric detection of a range of compounds that differ in both isometric structure and N-alkyl chain length. Although column retention appears dependent upon residual charge on the imine function, successful isocratic separation can be achieved by secondary hydrophobic interactions. A series of N-alkylated deoxynojirimycin compounds containing C(1-10) alkyl chains are readily separated and detected by pulsed amperometry after cation suppression. Using experimentally derived response factors for imino sugars and measurement of peak areas we have developed a reliable method for quantitatively determining concentrations in solution. A rapid protocol for the removal of protein and contaminants in biological samples is described. This has allowed the successful measurement of imino sugars in animal tissues and will be useful for understanding the factors involved in compound bioavailability and in the design of novel therapeutics. |
| first_indexed | 2024-03-06T21:05:30Z |
| format | Journal article |
| id | oxford-uuid:3c4fda99-a49c-4015-b43d-b42a0c857cda |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:05:30Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:3c4fda99-a49c-4015-b43d-b42a0c857cda2022-03-26T14:12:53ZHigh-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3c4fda99-a49c-4015-b43d-b42a0c857cdaEnglishSymplectic Elements at Oxford2000Mellor, HAdam, APlatt, FDwek, RButters, TThe use of imino sugars for the potential treatment of lysosomal glycolipid storage diseases and hepatitis virus infections requires accurate, quantitative measurement of these compounds in biological samples. We demonstrate here the versatility of cation-exchange chromatography and pulsed amperometric detection of a range of compounds that differ in both isometric structure and N-alkyl chain length. Although column retention appears dependent upon residual charge on the imine function, successful isocratic separation can be achieved by secondary hydrophobic interactions. A series of N-alkylated deoxynojirimycin compounds containing C(1-10) alkyl chains are readily separated and detected by pulsed amperometry after cation suppression. Using experimentally derived response factors for imino sugars and measurement of peak areas we have developed a reliable method for quantitatively determining concentrations in solution. A rapid protocol for the removal of protein and contaminants in biological samples is described. This has allowed the successful measurement of imino sugars in animal tissues and will be useful for understanding the factors involved in compound bioavailability and in the design of novel therapeutics. |
| spellingShingle | Mellor, H Adam, A Platt, F Dwek, R Butters, T High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title | High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title_full | High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title_fullStr | High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title_full_unstemmed | High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title_short | High-performance cation-exchange chromatography and pulsed amperometric detection for the separation, detection, and quantitation of N-alkylated imino sugars in biological samples. |
| title_sort | high performance cation exchange chromatography and pulsed amperometric detection for the separation detection and quantitation of n alkylated imino sugars in biological samples |
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