Rapid and efficient DNA strand cross-linking by click chemistry.
Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much...
| Huvudupphovsmän: | , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2008
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| _version_ | 1826268214945906688 |
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| author | Kocalka, P El-Sagheer, A Brown, T |
| author_facet | Kocalka, P El-Sagheer, A Brown, T |
| author_sort | Kocalka, P |
| collection | OXFORD |
| description | Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min. |
| first_indexed | 2024-03-06T21:06:14Z |
| format | Journal article |
| id | oxford-uuid:3c8f401e-dcdc-4bc9-9702-7c0e809f728d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:06:14Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:3c8f401e-dcdc-4bc9-9702-7c0e809f728d2022-03-26T14:14:17ZRapid and efficient DNA strand cross-linking by click chemistry.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3c8f401e-dcdc-4bc9-9702-7c0e809f728dEnglishSymplectic Elements at Oxford2008Kocalka, PEl-Sagheer, ABrown, TClick chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min. |
| spellingShingle | Kocalka, P El-Sagheer, A Brown, T Rapid and efficient DNA strand cross-linking by click chemistry. |
| title | Rapid and efficient DNA strand cross-linking by click chemistry. |
| title_full | Rapid and efficient DNA strand cross-linking by click chemistry. |
| title_fullStr | Rapid and efficient DNA strand cross-linking by click chemistry. |
| title_full_unstemmed | Rapid and efficient DNA strand cross-linking by click chemistry. |
| title_short | Rapid and efficient DNA strand cross-linking by click chemistry. |
| title_sort | rapid and efficient dna strand cross linking by click chemistry |
| work_keys_str_mv | AT kocalkap rapidandefficientdnastrandcrosslinkingbyclickchemistry AT elsagheera rapidandefficientdnastrandcrosslinkingbyclickchemistry AT brownt rapidandefficientdnastrandcrosslinkingbyclickchemistry |