DySCo: Quantitating associations of membrane proteins using two-color single-molecule tracking

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capab...

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Chi tiết về thư mục
Những tác giả chính: Dunne, P, Fernandes, R, McColl, J, Ji, W, James, JR, Davis, S, Klenerman, D
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 2009
Miêu tả
Tóm tắt:We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cytoskeleton, and it is applicable to situations where the number of molecules is small. © 2009 by the Biophysical Society.