RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model.
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight diffe...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826268294803357696 |
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| author | Hu, C van Dommelen, J van der Heijden, R Spijksma, G Reijmers, T Wang, M Slee, E Lu, X Xu, G van der Greef, J Hankemeier, T |
| author_facet | Hu, C van Dommelen, J van der Heijden, R Spijksma, G Reijmers, T Wang, M Slee, E Lu, X Xu, G van der Greef, J Hankemeier, T |
| author_sort | Hu, C |
| collection | OXFORD |
| description | A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. |
| first_indexed | 2024-03-06T21:07:28Z |
| format | Journal article |
| id | oxford-uuid:3cf7d87a-f54c-469e-a85e-f4ffee8f92f3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:07:28Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:3cf7d87a-f54c-469e-a85e-f4ffee8f92f32022-03-26T14:16:42ZRPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3cf7d87a-f54c-469e-a85e-f4ffee8f92f3EnglishSymplectic Elements at Oxford2008Hu, Cvan Dommelen, Jvan der Heijden, RSpijksma, GReijmers, TWang, MSlee, ELu, XXu, Gvan der Greef, JHankemeier, TA reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. |
| spellingShingle | Hu, C van Dommelen, J van der Heijden, R Spijksma, G Reijmers, T Wang, M Slee, E Lu, X Xu, G van der Greef, J Hankemeier, T RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title | RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title_full | RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title_fullStr | RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title_full_unstemmed | RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title_short | RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model. |
| title_sort | rplc ion trap ftms method for lipid profiling of plasma method validation and application to p53 mutant mouse model |
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