The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9.
Human histocompatibility leukocyte antigen E (HLA-E) and mouse major histocompatibility complex (MHC) class Ib antigen, Qa-1, share the same substitutions at two normally conserved positions 143 and 147, which are likely to affect binding of the C terminus of peptides. Qa-1 is able to bind a peptide...
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1797064056051335168 |
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| author | Braud, V Jones, E McMichael, A |
| author_facet | Braud, V Jones, E McMichael, A |
| author_sort | Braud, V |
| collection | OXFORD |
| description | Human histocompatibility leukocyte antigen E (HLA-E) and mouse major histocompatibility complex (MHC) class Ib antigen, Qa-1, share the same substitutions at two normally conserved positions 143 and 147, which are likely to affect binding of the C terminus of peptides. Qa-1 is able to bind a peptide derived from the leader sequence of H-2 D and H-2 L molecules. We developed a peptide binding assay in vitro to compare the binding specificity of HLA-E with the mouse MHC class Ib molecule Qa-1. We demonstrate that HLA-E binds, although poorly, the peptide which binds to Qa-1 and that it also binds nonamer signal sequence-derived peptides from human MHC class I molecules. Using alanine and glycine substitutions, we could define primary anchor residues at positions 2 and 9 and secondary anchor residues at position 7 and possibly 3. |
| first_indexed | 2024-03-06T21:08:49Z |
| format | Journal article |
| id | oxford-uuid:3d6ef8d4-f185-4943-a19a-23cde695ebe4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:08:49Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:3d6ef8d4-f185-4943-a19a-23cde695ebe42022-03-26T14:19:17ZThe human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3d6ef8d4-f185-4943-a19a-23cde695ebe4EnglishSymplectic Elements at Oxford1997Braud, VJones, EMcMichael, AHuman histocompatibility leukocyte antigen E (HLA-E) and mouse major histocompatibility complex (MHC) class Ib antigen, Qa-1, share the same substitutions at two normally conserved positions 143 and 147, which are likely to affect binding of the C terminus of peptides. Qa-1 is able to bind a peptide derived from the leader sequence of H-2 D and H-2 L molecules. We developed a peptide binding assay in vitro to compare the binding specificity of HLA-E with the mouse MHC class Ib molecule Qa-1. We demonstrate that HLA-E binds, although poorly, the peptide which binds to Qa-1 and that it also binds nonamer signal sequence-derived peptides from human MHC class I molecules. Using alanine and glycine substitutions, we could define primary anchor residues at positions 2 and 9 and secondary anchor residues at position 7 and possibly 3. |
| spellingShingle | Braud, V Jones, E McMichael, A The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title | The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title_full | The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title_fullStr | The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title_full_unstemmed | The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title_short | The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. |
| title_sort | human major histocompatibility complex class ib molecule hla e binds signal sequence derived peptides with primary anchor residues at positions 2 and 9 |
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