Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response.
Cytotoxic T lymphocytes (CTL) can respond to a few viral peptide-MHC-I (pMHC-I) complexes among a myriad of virus-unrelated endogenous self pMHC-I complexes displayed on virus-infected cells. To elucidate the molecular recognition events on live CTL, we have utilized a self-assembled biosensor compo...
| Κύριοι συγγραφείς: | , , , , , , |
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| Μορφή: | Journal article |
| Γλώσσα: | English |
| Έκδοση: |
2006
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| _version_ | 1826268390058098688 |
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| author | Anikeeva, N Lebedeva, T Clapp, A Goldman, E Dustin, M Mattoussi, H Sykulev, Y |
| author_facet | Anikeeva, N Lebedeva, T Clapp, A Goldman, E Dustin, M Mattoussi, H Sykulev, Y |
| author_sort | Anikeeva, N |
| collection | OXFORD |
| description | Cytotoxic T lymphocytes (CTL) can respond to a few viral peptide-MHC-I (pMHC-I) complexes among a myriad of virus-unrelated endogenous self pMHC-I complexes displayed on virus-infected cells. To elucidate the molecular recognition events on live CTL, we have utilized a self-assembled biosensor composed of semiconductor nanocrystals, quantum dots, carrying a controlled number of virus-derived (cognate) and other (noncognate) pMHC-I complexes and examined their recognition by antigen-specific T cell receptor (TCR) on anti-virus CD8(+) T cells. The unique architecture of nanoscale quantum dot/pMHC-I conjugates revealed that unexpectedly strong multivalent CD8-MHC-I interactions underlie the cooperative contribution of noncognate pMHC-I to the recognition of cognate pMHC-I by TCR to augment T cell responses. The cooperative, CD8-dependent spread of signal from a few productively engaged TCR to many other TCR can explain the remarkable ability of CTL to respond to virus-infected cells that present few cognate pMHC-I complexes. |
| first_indexed | 2024-03-06T21:08:56Z |
| format | Journal article |
| id | oxford-uuid:3d7a78d9-5bcb-41aa-a435-f5bdd5de567a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:08:56Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:3d7a78d9-5bcb-41aa-a435-f5bdd5de567a2022-03-26T14:19:36ZQuantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3d7a78d9-5bcb-41aa-a435-f5bdd5de567aEnglishSymplectic Elements at Oxford2006Anikeeva, NLebedeva, TClapp, AGoldman, EDustin, MMattoussi, HSykulev, YCytotoxic T lymphocytes (CTL) can respond to a few viral peptide-MHC-I (pMHC-I) complexes among a myriad of virus-unrelated endogenous self pMHC-I complexes displayed on virus-infected cells. To elucidate the molecular recognition events on live CTL, we have utilized a self-assembled biosensor composed of semiconductor nanocrystals, quantum dots, carrying a controlled number of virus-derived (cognate) and other (noncognate) pMHC-I complexes and examined their recognition by antigen-specific T cell receptor (TCR) on anti-virus CD8(+) T cells. The unique architecture of nanoscale quantum dot/pMHC-I conjugates revealed that unexpectedly strong multivalent CD8-MHC-I interactions underlie the cooperative contribution of noncognate pMHC-I to the recognition of cognate pMHC-I by TCR to augment T cell responses. The cooperative, CD8-dependent spread of signal from a few productively engaged TCR to many other TCR can explain the remarkable ability of CTL to respond to virus-infected cells that present few cognate pMHC-I complexes. |
| spellingShingle | Anikeeva, N Lebedeva, T Clapp, A Goldman, E Dustin, M Mattoussi, H Sykulev, Y Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title | Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title_full | Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title_fullStr | Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title_full_unstemmed | Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title_short | Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. |
| title_sort | quantum dot peptide mhc biosensors reveal strong cd8 dependent cooperation between self and viral antigens that augment the t cell response |
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