Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching.
Subdiffraction fluorescence imaging is presented in a parallelized wide-field arrangement exploiting the principle of reversible saturable/switchable optical transitions (RESOLFT). The diffraction barrier is overcome by photoswitching ensembles of the label protein asFP595 between a nonfluorescent o...
| Autores principales: | , , , , , , |
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| Formato: | Journal article |
| Lenguaje: | English |
| Publicado: |
2007
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