SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation.
SpoIIIE/FtsK are membrane-anchored, ATP-fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence-recognition modules (SpoIIIE-γ/FtsK-γ) and highly skewed chromosomal sequences (SRS/KOPS)....
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797064153146327040 |
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| author | Cattoni, D Chara, O Godefroy, C Margeat, E Trigueros, S Milhiet, P Nöllmann, M |
| author_facet | Cattoni, D Chara, O Godefroy, C Margeat, E Trigueros, S Milhiet, P Nöllmann, M |
| author_sort | Cattoni, D |
| collection | OXFORD |
| description | SpoIIIE/FtsK are membrane-anchored, ATP-fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence-recognition modules (SpoIIIE-γ/FtsK-γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single-molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single-molecule and modelling methods that hexameric SpoIIIE binds DNA non-specifically and finds SRS by an ATP-independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE-γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS-directed translocation by SpoIIIE/FtsK. |
| first_indexed | 2024-03-06T21:10:10Z |
| format | Journal article |
| id | oxford-uuid:3de06b3c-b03d-41a1-8856-5eb7699d7dcf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:10:10Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:3de06b3c-b03d-41a1-8856-5eb7699d7dcf2022-03-26T14:22:04ZSpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3de06b3c-b03d-41a1-8856-5eb7699d7dcfEnglishSymplectic Elements at Oxford2013Cattoni, DChara, OGodefroy, CMargeat, ETrigueros, SMilhiet, PNöllmann, MSpoIIIE/FtsK are membrane-anchored, ATP-fuelled, directional motors responsible for chromosomal segregation in bacteria. Directionality in these motors is governed by interactions between specialized sequence-recognition modules (SpoIIIE-γ/FtsK-γ) and highly skewed chromosomal sequences (SRS/KOPS). Using a new combination of ensemble and single-molecule methods, we dissect the series of steps required for SRS localization and motor activation. First, we demonstrate that SpoIIIE/DNA association kinetics are sequence independent, with binding specificity being uniquely determined by dissociation. Next, we show by single-molecule and modelling methods that hexameric SpoIIIE binds DNA non-specifically and finds SRS by an ATP-independent target search mechanism, with ensuing oligomerization and binding of SpoIIIE-γ to SRS triggering motor stimulation. Finally, we propose a new model that provides an entirely new interpretation of previous observations for the origin of SRS/KOPS-directed translocation by SpoIIIE/FtsK. |
| spellingShingle | Cattoni, D Chara, O Godefroy, C Margeat, E Trigueros, S Milhiet, P Nöllmann, M SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title | SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title_full | SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title_fullStr | SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title_full_unstemmed | SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title_short | SpoIIIE mechanism of directional translocation involves target search coupled to sequence-dependent motor stimulation. |
| title_sort | spoiiie mechanism of directional translocation involves target search coupled to sequence dependent motor stimulation |
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