Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis
<strong>Introduction</strong> Previous studies have shown increased expression of stromal markers in synovial tissue (ST) of patients with established rheumatoid arthritis (RA). Here, ST expression of stromal markers in early arthritis in relationship to diagnosis and prognostic outcome...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Published: |
Public Library of Science
2017
|
| _version_ | 1826268508057501696 |
|---|---|
| author | Choi, I Karpus, O Turner, J Hardie, D Marshall, J de Hair, M Maijer, K Tak, P Raza, K Hamann, J Buckley, C Gerlag, D Filer, A |
| author_facet | Choi, I Karpus, O Turner, J Hardie, D Marshall, J de Hair, M Maijer, K Tak, P Raza, K Hamann, J Buckley, C Gerlag, D Filer, A |
| author_sort | Choi, I |
| collection | OXFORD |
| description | <strong>Introduction</strong> Previous studies have shown increased expression of stromal markers in synovial tissue (ST) of patients with established rheumatoid arthritis (RA). Here, ST expression of stromal markers in early arthritis in relationship to diagnosis and prognostic outcome was studied. <strong>Methods</strong> ST from 56 patients included in two different early arthritis cohorts and 7 non-inflammatory controls was analysed using immunofluorescence to detect stromal markers CD55, CD248, fibroblast activation protein (FAP) and podoplanin. Diagnostic classification (gout, psoriatic arthritis, unclassified arthritis (UA), parvovirus associated arthritis, reactive arthritis and RA), disease outcome (resolving vs persistent) and clinical variables were determined at baseline and after follow-up, and related to the expression of stromal markers. <strong>Results</strong> We observed expression of all stromal markers in ST of early arthritis patients, independent of diagnosis or prognostic outcome. Synovial expression of FAP was significantly higher in patients developing early RA compared to other diagnostic groups and non-inflammatory controls. In RA FAP protein was expressed in both lining and sublining layers. Podoplanin expression was higher in all early inflammatory arthritis patients than controls, but did not differentiate diagnostic outcomes. Stromal marker expression was not associated with prognostic outcomes of disease persistence or resolution. There was no association with clinical or sonographic variables. <strong>Conclusions</strong> Stromal cell markers CD55, CD248, FAP and podoplanin are expressed in ST in the earliest stage of arthritis. Baseline expression of FAP is higher in early synovitis patients who fulfil classification criteria for RA over time. These results suggest that significant fibroblast activation occurs in RA in the early window of disease. |
| first_indexed | 2024-03-06T21:10:45Z |
| format | Journal article |
| id | oxford-uuid:3e11667e-1fd8-4d04-a7e7-613b0197ab32 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T21:10:45Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:3e11667e-1fd8-4d04-a7e7-613b0197ab322022-03-26T14:23:14ZStromal cell markers are differentially expressed in the synovial tissue of patients with early arthritisJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3e11667e-1fd8-4d04-a7e7-613b0197ab32Symplectic Elements at OxfordPublic Library of Science2017Choi, IKarpus, OTurner, JHardie, DMarshall, Jde Hair, MMaijer, KTak, PRaza, KHamann, JBuckley, CGerlag, DFiler, A<strong>Introduction</strong> Previous studies have shown increased expression of stromal markers in synovial tissue (ST) of patients with established rheumatoid arthritis (RA). Here, ST expression of stromal markers in early arthritis in relationship to diagnosis and prognostic outcome was studied. <strong>Methods</strong> ST from 56 patients included in two different early arthritis cohorts and 7 non-inflammatory controls was analysed using immunofluorescence to detect stromal markers CD55, CD248, fibroblast activation protein (FAP) and podoplanin. Diagnostic classification (gout, psoriatic arthritis, unclassified arthritis (UA), parvovirus associated arthritis, reactive arthritis and RA), disease outcome (resolving vs persistent) and clinical variables were determined at baseline and after follow-up, and related to the expression of stromal markers. <strong>Results</strong> We observed expression of all stromal markers in ST of early arthritis patients, independent of diagnosis or prognostic outcome. Synovial expression of FAP was significantly higher in patients developing early RA compared to other diagnostic groups and non-inflammatory controls. In RA FAP protein was expressed in both lining and sublining layers. Podoplanin expression was higher in all early inflammatory arthritis patients than controls, but did not differentiate diagnostic outcomes. Stromal marker expression was not associated with prognostic outcomes of disease persistence or resolution. There was no association with clinical or sonographic variables. <strong>Conclusions</strong> Stromal cell markers CD55, CD248, FAP and podoplanin are expressed in ST in the earliest stage of arthritis. Baseline expression of FAP is higher in early synovitis patients who fulfil classification criteria for RA over time. These results suggest that significant fibroblast activation occurs in RA in the early window of disease. |
| spellingShingle | Choi, I Karpus, O Turner, J Hardie, D Marshall, J de Hair, M Maijer, K Tak, P Raza, K Hamann, J Buckley, C Gerlag, D Filer, A Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title | Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title_full | Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title_fullStr | Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title_full_unstemmed | Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title_short | Stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| title_sort | stromal cell markers are differentially expressed in the synovial tissue of patients with early arthritis |
| work_keys_str_mv | AT choii stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT karpuso stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT turnerj stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT hardied stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT marshallj stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT dehairm stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT maijerk stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT takp stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT razak stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT hamannj stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT buckleyc stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT gerlagd stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis AT filera stromalcellmarkersaredifferentiallyexpressedinthesynovialtissueofpatientswithearlyarthritis |