Multiplexed analysis of chromosome conformation at vastly improved sensitivity

Methods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C ca...

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मुख्य लेखकों: Davies, J, Telenius, J, McGowan, S, Roberts, N, Taylor, S, Higgs, D, Hughes, J
स्वरूप: Journal article
भाषा:English
प्रकाशित: Nature Research 2015
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author Davies, J
Telenius, J
McGowan, S
Roberts, N
Taylor, S
Higgs, D
Hughes, J
author_facet Davies, J
Telenius, J
McGowan, S
Roberts, N
Taylor, S
Higgs, D
Hughes, J
author_sort Davies, J
collection OXFORD
description Methods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C can be used to analyze many genetic loci and samples simultaneously. High-resolution data can be produced with as few as 100,000 cells, and single-nucleotide polymorphisms can be used to generate allele-specific tracks. The method is straightforward to perform and should greatly facilitate the investigation of many questions related to gene regulation as well as the functional dissection of traits examined in genome-wide association studies.
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spelling oxford-uuid:3e47a1e7-9767-4fb1-93fe-199a788f35432022-03-26T14:24:33ZMultiplexed analysis of chromosome conformation at vastly improved sensitivityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3e47a1e7-9767-4fb1-93fe-199a788f3543EnglishSymplectic Elements at OxfordNature Research2015Davies, JTelenius, JMcGowan, SRoberts, NTaylor, SHiggs, DHughes, JMethods for analyzing chromosome conformation in mammalian cells are either low resolution or low throughput and are technically challenging. In next-generation (NG) Capture-C, we have redesigned the Capture-C method to achieve unprecedented levels of sensitivity and reproducibility. NG Capture-C can be used to analyze many genetic loci and samples simultaneously. High-resolution data can be produced with as few as 100,000 cells, and single-nucleotide polymorphisms can be used to generate allele-specific tracks. The method is straightforward to perform and should greatly facilitate the investigation of many questions related to gene regulation as well as the functional dissection of traits examined in genome-wide association studies.
spellingShingle Davies, J
Telenius, J
McGowan, S
Roberts, N
Taylor, S
Higgs, D
Hughes, J
Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title_full Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title_fullStr Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title_full_unstemmed Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title_short Multiplexed analysis of chromosome conformation at vastly improved sensitivity
title_sort multiplexed analysis of chromosome conformation at vastly improved sensitivity
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