Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio
Objective:We hypothesized that genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits.Methods:Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3 and GRB14) withi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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2014
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| _version_ | 1797064235373559808 |
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| author | Schleinitz, D Kloeting, N Lindgren, C Breitfeld, J Dietrich, A Schoen, MR Lohmann, T Dressler, M Stumvoll, M McCarthy, M Blueher, M Kovacs, P |
| author_facet | Schleinitz, D Kloeting, N Lindgren, C Breitfeld, J Dietrich, A Schoen, MR Lohmann, T Dressler, M Stumvoll, M McCarthy, M Blueher, M Kovacs, P |
| author_sort | Schleinitz, D |
| collection | OXFORD |
| description | Objective:We hypothesized that genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits.Methods:Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3 and GRB14) within these loci showing coincident cis-expression quantitative trait loci was measured in 222 paired samples of human visceral (vis) and subcutaneous (sc) AT. The relationship of mRNA expression levels with obesity-related quantitative traits was assessed by Pearson's correlation analyses. Multivariate linear relationships were assessed by generalized linear regression models.Results:Whereas only PIGC, ZNFR3 and STAB1 mRNA expression in sc AT correlated nominally with WHR (P<0.05, adjusted for age and sex), mRNA expression of all studied genes in at least one of the fat depots correlated significantly with vis and/or sc fat area (P ranging from 0.05 to 4.0 × 106, adjusted for age and sex). Consistently, the transcript levels of WARS, PIGC and GRB14 were nominally associated with body mass index (BMI) (P ranging from 0.02 to 9.2 × 105, adjusted for age and sex). Moreover, independent of sex, obesity and diabetes status, differential expression between vis and sc AT was observed for all tested genes (P<0.01). Finally, the rs10195252 T-allele was nominally associated with increased GRB14 sc mRNA expression (P=0.025 after adjusting for age, sex and BMI).Conclusions:Our data including the inter-depot variability of mRNA expression suggests that genes within the WHR-associated loci might be involved in the regulation of fat distribution. © 2014 Macmillan Publishers Limited. |
| first_indexed | 2024-03-06T21:11:21Z |
| format | Journal article |
| id | oxford-uuid:3e48cb4f-9c9f-4235-a5bb-d7f8fb3ad16b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:11:21Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:3e48cb4f-9c9f-4235-a5bb-d7f8fb3ad16b2022-03-26T14:24:34ZFat depot-specific mRNA expression of novel loci associated with waist-hip ratioJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3e48cb4f-9c9f-4235-a5bb-d7f8fb3ad16bEnglishSymplectic Elements at Oxford2014Schleinitz, DKloeting, NLindgren, CBreitfeld, JDietrich, ASchoen, MRLohmann, TDressler, MStumvoll, MMcCarthy, MBlueher, MKovacs, PObjective:We hypothesized that genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits.Methods:Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3 and GRB14) within these loci showing coincident cis-expression quantitative trait loci was measured in 222 paired samples of human visceral (vis) and subcutaneous (sc) AT. The relationship of mRNA expression levels with obesity-related quantitative traits was assessed by Pearson's correlation analyses. Multivariate linear relationships were assessed by generalized linear regression models.Results:Whereas only PIGC, ZNFR3 and STAB1 mRNA expression in sc AT correlated nominally with WHR (P<0.05, adjusted for age and sex), mRNA expression of all studied genes in at least one of the fat depots correlated significantly with vis and/or sc fat area (P ranging from 0.05 to 4.0 × 106, adjusted for age and sex). Consistently, the transcript levels of WARS, PIGC and GRB14 were nominally associated with body mass index (BMI) (P ranging from 0.02 to 9.2 × 105, adjusted for age and sex). Moreover, independent of sex, obesity and diabetes status, differential expression between vis and sc AT was observed for all tested genes (P<0.01). Finally, the rs10195252 T-allele was nominally associated with increased GRB14 sc mRNA expression (P=0.025 after adjusting for age, sex and BMI).Conclusions:Our data including the inter-depot variability of mRNA expression suggests that genes within the WHR-associated loci might be involved in the regulation of fat distribution. © 2014 Macmillan Publishers Limited. |
| spellingShingle | Schleinitz, D Kloeting, N Lindgren, C Breitfeld, J Dietrich, A Schoen, MR Lohmann, T Dressler, M Stumvoll, M McCarthy, M Blueher, M Kovacs, P Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title | Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title_full | Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title_fullStr | Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title_full_unstemmed | Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title_short | Fat depot-specific mRNA expression of novel loci associated with waist-hip ratio |
| title_sort | fat depot specific mrna expression of novel loci associated with waist hip ratio |
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