Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.

A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective wi...

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मुख्य लेखकों: May, J, Brown, L, van Delft, I, Thelwell, N, Harley, K, Brown, T
स्वरूप: Journal article
भाषा:English
प्रकाशित: 2005
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author May, J
Brown, L
van Delft, I
Thelwell, N
Harley, K
Brown, T
author_facet May, J
Brown, L
van Delft, I
Thelwell, N
Harley, K
Brown, T
author_sort May, J
collection OXFORD
description A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective with a wide range of fluorescent dyes. The anthraquinone moiety increases the melting temperature of DNA duplexes, thus allowing shorter, more discriminatory probes to be used. The quencher has been used in Scorpion primers and TaqMan probes for human DNA sequence recognition and mutation detection.
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spelling oxford-uuid:3f667847-a66e-47b4-8b57-82c72c3b6fdb2022-03-26T14:31:48ZSynthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3f667847-a66e-47b4-8b57-82c72c3b6fdbEnglishSymplectic Elements at Oxford2005May, JBrown, Lvan Delft, IThelwell, NHarley, KBrown, TA non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective with a wide range of fluorescent dyes. The anthraquinone moiety increases the melting temperature of DNA duplexes, thus allowing shorter, more discriminatory probes to be used. The quencher has been used in Scorpion primers and TaqMan probes for human DNA sequence recognition and mutation detection.
spellingShingle May, J
Brown, L
van Delft, I
Thelwell, N
Harley, K
Brown, T
Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title_full Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title_fullStr Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title_full_unstemmed Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title_short Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
title_sort synthesis and evaluation of a new non fluorescent quencher in fluorogenic oligonucleotide probes for real time pcr
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