Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.

A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective wi...

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Bibliografiset tiedot
Päätekijät: May, J, Brown, L, van Delft, I, Thelwell, N, Harley, K, Brown, T
Aineistotyyppi: Journal article
Kieli:English
Julkaistu: 2005