Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.

Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.

Deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) is an iron(II) and 2-oxoglutarate-dependent oxygenase involved in the biosynthesis of cephalosporin C in Cephalosporium acremonium. It catalyzes two oxidative reactions, oxidative ring-expansion of penicillin N to deacetoxycephalosp...

Deskribapen osoa

Xehetasun bibliografikoak
Egile Nagusiak:	Lloyd, MD, Lipscomb, S, Hewitson, K, Hensgens, C, Baldwin, J, Schofield, C
Formatua:	Journal article
Hizkuntza:	English
Argitaratua:	2004

Antzeko izenburuak

Oxidation of deacetylcephalosporin C by deacetoxycephalosporin C/deacetylcephalosporin C synthase.
nork: Baldwin, J, et al.
Argitaratua: (1992)

Substrate specificity of cloned deacetoxycephalosporin C/deacetylcephalosporin C synthetase.
nork: Baldwin, J, et al.
Argitaratua: (1988)

The role of arginine residues in substrate binding and catalysis by deacetoxycephalosporin C synthase.
nork: Lipscomb, S, et al.
Argitaratua: (2002)

Adipoyl-6-aminopenicillanic acid is a substrate for deacetoxycephalosporin C synthase (DAOCS)
nork: Shibata, N, et al.
Argitaratua: (1996)

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase. The role of arginine 258.
nork: Lee, H, et al.
Argitaratua: (2001)

Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.
nork: Dubus, A, et al.
Argitaratua: (2001)

Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).
nork: Lee, H, et al.
Argitaratua: (2001)

Active site mutations of recombinant deacetoxycephalosporin C synthase.
nork: Lee, H, et al.
Argitaratua: (2002)

Studies on the active site of deacetoxycephalosporin C synthase.
nork: Lloyd, MD, et al.
Argitaratua: (1999)

Studies on deacetoxy/deacetylcephalosporin C synthase
nork: Pereira, I, et al.
Argitaratua: (1993)

The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S. clavuligerus.
nork: Lee, H, et al.
Argitaratua: (2000)

The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase.
nork: Lee, H, et al.
Argitaratua: (2003)

STUDIES ON THE MECHANISM OF DEACETOXY DEACETYLCEPHALOSPORIN-C SYNTHASE USING CYCLOPROPYL SUBSTITUTED CEPHALOSPORIN PROBES
nork: Baldwin, J, et al.
Argitaratua: (1991)

Studies on deacetoxycephalosporin C synthase support a consensus mechanism for 2-oxoglutarate dependent oxygenases.
nork: Tarhonskaya, H, et al.
Argitaratua: (2014)

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.
nork: Roach, P, et al.
Argitaratua: (1997)

SUBSTRATE-ANALOG STUDIES ON CLAVAMINIC ACID SYNTHASE
nork: Baldwin, J, et al.
Argitaratua: (1993)

STEPWISE HYDROGEN REMOVAL IN THE ENZYMATIC RING EXPANSION OF PENICILLIN-N TO DEACETOXYCEPHALOSPORIN-C
nork: Baldwin, J, et al.
Argitaratua: (1987)

Purification and initial characterization of an enzyme with deacetoxycephalosporin C synthetase and hydroxylase activities.
nork: Baldwin, J, et al.
Argitaratua: (1987)

Anaerobic crystallisation of an isopenicillin N synthase.Fe(II).substrate complex demonstrated by X-ray studies.
nork: Roach, P, et al.
Argitaratua: (1996)

The C-4 stereochemistry of leucocyanidin substrates for anthocyanidin synthase affects product selectivity.
nork: Turnbull, J, et al.
Argitaratua: (2003)

Product-substrate engineering by bacteria: Studies on clavaminate synthase, a trifunctional dioxygenase
nork: Lloyd, MD, et al.
Argitaratua: (1999)

Anaerobic crystallisation of an isopenicillin N synthase center dot Fe(II)center dot substrate complex demonstrated by X-ray studies
nork: Roach, P, et al.
Argitaratua: (1996)

A SUBSTRATE-ANALOG STUDY ON CLAVAMINIC ACID SYNTHASE - POSSIBLE CLUES TO THE BIOSYNTHETIC ORIGIN OF PROCLAVAMIC ACID
nork: Baldwin, J, et al.
Argitaratua: (1993)

The mechanism of the enzymatic ring expansion of penicillin N to deacetoxycephalosporin C
nork: Crouch, N, et al.
Argitaratua: (1988)

Biosynthesis of carbapenem antibiotics: new carbapenam substrates for carbapenem synthase (CarC).
nork: Sleeman, M, et al.
Argitaratua: (2004)

Structural and mechanistic studies on anthocyanidin synthase catalysed oxidation of flavanone substrates: the effect of C-2 stereochemistry on product selectivity and mechanism.
nork: Welford, R, et al.
Argitaratua: (2005)

Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase.
nork: Zhang, Z, et al.
Argitaratua: (2000)

Mutagenesis studies on the iron binding ligands of clavaminic acid synthase.
nork: Doan, L, et al.
Argitaratua: (2000)

Crystallographic studies on the binding of selectively deuterated LLD- and LLL-substrate epimers by isopenicillin N synthase.
nork: Ge, W, et al.
Argitaratua: (2010)

EXAFS STUDIES OF ISOPENICILLIN-N SYNTHASE
nork: Randall, C, et al.
Argitaratua: (1992)

Structure of a cephalosporin synthase.
nork: Valegård, K, et al.
Argitaratua: (1998)

Substrate and metal ion promiscuity in mannosylglycerate synthase.
nork: Nielsen, M, et al.
Argitaratua: (2011)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
nork: Baldwin, J, et al.
Argitaratua: (1991)

ISOPENICILLIN-N SYNTHASE - A NEW MODE OF REACTIVITY
nork: Baldwin, J, et al.
Argitaratua: (1992)

Substrate recognition by holocytochrome c synthase in cytochrome c biogenesis system III
nork: Zhang, Y
Argitaratua: (2015)

Chemo-enzymatic synthesis of bicyclic gamma-lactams using clavaminic acid synthase
nork: Baldwin, J, et al.
Argitaratua: (1997)

The crystal structure of isopenicillin N synthase with a dipeptide substrate analogue.
nork: Daruzzaman, A, et al.
Argitaratua: (2013)

Structural studies on the reaction of isopenicillin N synthase with the substrate analogue delta-(l-alpha-aminoadipoyl)-l-cysteinyl-d-alpha-aminobutyrate.
nork: Long, A, et al.
Argitaratua: (2003)

ENZYMATIC-SYNTHESIS OF BICYCLIC GAMMA-LACTAMS USING CLAVAMINIC ACID SYNTHASE
nork: Baldwin, J, et al.
Argitaratua: (1992)

STUDIES ON THE STEREOSPECIFICITY OF THE CLAVAMINIC ACID SYNTHASE CATALYZED HYDROXYLATION REACTION
nork: Baldwin, J, et al.
Argitaratua: (1993)