Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum
Background: Antibodies that inhibit the growth of blood-stage <em>Plasmodium falciparum</em> may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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Public Library of Science
2008
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| _version_ | 1797064697082544128 |
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| author | McCallum, F Persson, K Mugyenyi, C Fowkes, F Simpson, J Richards, J Williams, T Marsh, K Beeson, J |
| author_facet | McCallum, F Persson, K Mugyenyi, C Fowkes, F Simpson, J Richards, J Williams, T Marsh, K Beeson, J |
| author_sort | McCallum, F |
| collection | OXFORD |
| description | Background: Antibodies that inhibit the growth of blood-stage <em>Plasmodium falciparum</em> may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum and purified immunoglobulins from Kenyan children and adults for inhibition of <em>P. falciparum</em> blood-stage growth <em>in vitro</em> using different parasite lines. Serum antibodies were measured by ELISA to bloodstage parasite antigens, extracted from <em>P. falciparum</em> schizonts, and to recombinant merozoite surface protein 1 (42 kDa Cterminal fragment, MSP1-42). Results: Antibodies to blood-stage antigens present in schizont protein extract and to recombinant MSP1-42 significantly increased with age and were highly correlated. In contrast, growth-inhibitory activity was not strongly associated with age and tended to decline marginally with increasing age and exposure, with young children demonstrating the highest inhibitory activity. Comparison of growth-inhibitory activity among samples collected from the same population at different time points suggested that malaria transmission intensity influenced the level of growth-inhibitory antibodies. Antibodies to recombinant MSP1-42 were not associated with growth inhibition and high immunoglobulin G levels were poorly predictive of inhibitory activity. The level of inhibitory activity against different isolates varied. Conclusions: Children can acquire growth-inhibitory antibodies at a young age, but once they are acquired they do not appear to be boosted by on-going exposure. Inhibitory antibodies may play a role in protection from early childhood malaria. |
| first_indexed | 2024-03-06T21:18:02Z |
| format | Journal article |
| id | oxford-uuid:40789924-d1cb-43d1-a9d0-e7eee322bbd3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:18:02Z |
| publishDate | 2008 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oxford-uuid:40789924-d1cb-43d1-a9d0-e7eee322bbd32022-03-26T14:38:09ZAcquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparumJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:40789924-d1cb-43d1-a9d0-e7eee322bbd3Medical sciencesMalariaEnglishOxford University Research Archive - ValetPublic Library of Science2008McCallum, FPersson, KMugyenyi, CFowkes, FSimpson, JRichards, JWilliams, TMarsh, KBeeson, JBackground: Antibodies that inhibit the growth of blood-stage <em>Plasmodium falciparum</em> may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum and purified immunoglobulins from Kenyan children and adults for inhibition of <em>P. falciparum</em> blood-stage growth <em>in vitro</em> using different parasite lines. Serum antibodies were measured by ELISA to bloodstage parasite antigens, extracted from <em>P. falciparum</em> schizonts, and to recombinant merozoite surface protein 1 (42 kDa Cterminal fragment, MSP1-42). Results: Antibodies to blood-stage antigens present in schizont protein extract and to recombinant MSP1-42 significantly increased with age and were highly correlated. In contrast, growth-inhibitory activity was not strongly associated with age and tended to decline marginally with increasing age and exposure, with young children demonstrating the highest inhibitory activity. Comparison of growth-inhibitory activity among samples collected from the same population at different time points suggested that malaria transmission intensity influenced the level of growth-inhibitory antibodies. Antibodies to recombinant MSP1-42 were not associated with growth inhibition and high immunoglobulin G levels were poorly predictive of inhibitory activity. The level of inhibitory activity against different isolates varied. Conclusions: Children can acquire growth-inhibitory antibodies at a young age, but once they are acquired they do not appear to be boosted by on-going exposure. Inhibitory antibodies may play a role in protection from early childhood malaria. |
| spellingShingle | Medical sciences Malaria McCallum, F Persson, K Mugyenyi, C Fowkes, F Simpson, J Richards, J Williams, T Marsh, K Beeson, J Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title | Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title_full | Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title_fullStr | Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title_full_unstemmed | Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title_short | Acquisition of growth-inhibitory antibodies against blood-stage Plasmodium falciparum |
| title_sort | acquisition of growth inhibitory antibodies against blood stage plasmodium falciparum |
| topic | Medical sciences Malaria |
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