Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.
Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-h...
| Glavni autori: | , , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2001
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| _version_ | 1826269035995594752 |
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| author | Kershaw, N Mukherji, M MacKinnon, C Claridge, T Odell, B Wierzbicki, A Lloyd, MD Schofield, C |
| author_facet | Kershaw, N Mukherji, M MacKinnon, C Claridge, T Odell, B Wierzbicki, A Lloyd, MD Schofield, C |
| author_sort | Kershaw, N |
| collection | OXFORD |
| description | Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active. |
| first_indexed | 2024-03-06T21:18:46Z |
| format | Journal article |
| id | oxford-uuid:40bb05a0-0498-4295-8fb4-e966646e5629 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:18:46Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:40bb05a0-0498-4295-8fb4-e966646e56292022-03-26T14:39:27ZStudies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:40bb05a0-0498-4295-8fb4-e966646e5629EnglishSymplectic Elements at Oxford2001Kershaw, NMukherji, MMacKinnon, CClaridge, TOdell, BWierzbicki, ALloyd, MDSchofield, CPhytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active. |
| spellingShingle | Kershaw, N Mukherji, M MacKinnon, C Claridge, T Odell, B Wierzbicki, A Lloyd, MD Schofield, C Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title | Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title_full | Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title_fullStr | Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title_full_unstemmed | Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title_short | Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. |
| title_sort | studies on phytanoyl coa 2 hydroxylase and synthesis of phytanoyl coenzyme a |
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