Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours.
Dentin matrix protein 1 (DMP-1) is highly expressed by osteocytes and is a non-collagenous matrix protein found in dentin and bone. In this study, we determined the expression of DMP-1 in mature and immature human bone and examined whether DMP-1 is useful in distinguishing osteoid/bone-forming tumou...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2013
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| _version_ | 1826269134961246208 |
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| author | Kashima, T Dongre, A Oppermann, U Athanasou, N |
| author_facet | Kashima, T Dongre, A Oppermann, U Athanasou, N |
| author_sort | Kashima, T |
| collection | OXFORD |
| description | Dentin matrix protein 1 (DMP-1) is highly expressed by osteocytes and is a non-collagenous matrix protein found in dentin and bone. In this study, we determined the expression of DMP-1 in mature and immature human bone and examined whether DMP-1 is useful in distinguishing osteoid/bone-forming tumours from other primary and secondary bone tumours. DMP-1 expression was immunohistochemically determined in paraffin sections of a wide range of benign and malignant primary bone tumours and tumour-like lesions (n = 353). DMP-1 mRNA expression was also examined in osteosarcoma and fibrosarcoma cell lines as well as bone tumour specimens (n = 5) using real-time PCR. In lamellar and woven bone, DMP-1 was expressed in the matrix around osteocyte lacunae and canaliculi; osteoblasts and other cell types in the bone were negative. Matrix staining of the osteoid and bone was seen in bone-forming tumours including osteoma, osteoid osteoma, osteoblastoma and osteosarcoma. DMP-1 staining was also seen in fibrous dysplasia, osteofibrous dysplasia and chondroblastoma and in reactive bone in solitary bone cysts and aneurysmal bone cysts. DMP-1 was not expressed in the tumour component of other bone neoplasms including Ewing sarcoma, chondrosarcoma, leiomyosarcoma, fibrosarcoma, giant cell tumour of bone and metastatic carcinoma. DMP-1 mRNA was expressed in osteosarcoma cell lines and tumour samples. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours. |
| first_indexed | 2024-03-06T21:20:19Z |
| format | Journal article |
| id | oxford-uuid:413b9956-42cd-409a-a204-59c4dca5409d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:20:19Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:413b9956-42cd-409a-a204-59c4dca5409d2022-03-26T14:42:23ZDentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:413b9956-42cd-409a-a204-59c4dca5409dEnglishSymplectic Elements at Oxford2013Kashima, TDongre, AOppermann, UAthanasou, NDentin matrix protein 1 (DMP-1) is highly expressed by osteocytes and is a non-collagenous matrix protein found in dentin and bone. In this study, we determined the expression of DMP-1 in mature and immature human bone and examined whether DMP-1 is useful in distinguishing osteoid/bone-forming tumours from other primary and secondary bone tumours. DMP-1 expression was immunohistochemically determined in paraffin sections of a wide range of benign and malignant primary bone tumours and tumour-like lesions (n = 353). DMP-1 mRNA expression was also examined in osteosarcoma and fibrosarcoma cell lines as well as bone tumour specimens (n = 5) using real-time PCR. In lamellar and woven bone, DMP-1 was expressed in the matrix around osteocyte lacunae and canaliculi; osteoblasts and other cell types in the bone were negative. Matrix staining of the osteoid and bone was seen in bone-forming tumours including osteoma, osteoid osteoma, osteoblastoma and osteosarcoma. DMP-1 staining was also seen in fibrous dysplasia, osteofibrous dysplasia and chondroblastoma and in reactive bone in solitary bone cysts and aneurysmal bone cysts. DMP-1 was not expressed in the tumour component of other bone neoplasms including Ewing sarcoma, chondrosarcoma, leiomyosarcoma, fibrosarcoma, giant cell tumour of bone and metastatic carcinoma. DMP-1 mRNA was expressed in osteosarcoma cell lines and tumour samples. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours. |
| spellingShingle | Kashima, T Dongre, A Oppermann, U Athanasou, N Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title | Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title_full | Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title_fullStr | Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title_full_unstemmed | Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title_short | Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours. |
| title_sort | dentine matrix protein 1 dmp 1 is a marker of bone forming tumours |
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