Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells.
The cytosolic Ca2+ activity of mouse pancreatic beta-cells was studied with the intracellular fluorescent indicator quin2 . When the extracellular Ca2+ concentration was 1.20 mM, the basal cytosolic Ca2+ activity was 162 +/- 9 nM. Stimulation with 20 mM glucose increased this Ca2+ activity by 40%. I...
| প্রধান লেখক: | , , , |
|---|---|
| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
1984
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| _version_ | 1826269168421306368 |
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| author | Rorsman, P Abrahamsson, H Gylfe, E Hellman, B |
| author_facet | Rorsman, P Abrahamsson, H Gylfe, E Hellman, B |
| author_sort | Rorsman, P |
| collection | OXFORD |
| description | The cytosolic Ca2+ activity of mouse pancreatic beta-cells was studied with the intracellular fluorescent indicator quin2 . When the extracellular Ca2+ concentration was 1.20 mM, the basal cytosolic Ca2+ activity was 162 +/- 9 nM. Stimulation with 20 mM glucose increased this Ca2+ activity by 40%. In the presence of only 0.20 mM Ca2+ or after the addition of the voltage-dependent Ca2+ -channel blocker D-600, glucose had an opposite and more prompt effect in reducing cytosolic Ca2+ by about 15%. It is concluded that an early result of glucose exposure is a lowering of the cytosolic Ca2+ activity and that this effect tends to be masked by a subsequent increase of the Ca2+ activity due to influx of Ca2+ through the voltage-dependent Ca2+ channels. |
| first_indexed | 2024-03-06T21:20:50Z |
| format | Journal article |
| id | oxford-uuid:4164f02a-fe9e-4dde-8aa5-27e62c9c0fe9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:20:50Z |
| publishDate | 1984 |
| record_format | dspace |
| spelling | oxford-uuid:4164f02a-fe9e-4dde-8aa5-27e62c9c0fe92022-03-26T14:43:23ZDual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:4164f02a-fe9e-4dde-8aa5-27e62c9c0fe9EnglishSymplectic Elements at Oxford1984Rorsman, PAbrahamsson, HGylfe, EHellman, BThe cytosolic Ca2+ activity of mouse pancreatic beta-cells was studied with the intracellular fluorescent indicator quin2 . When the extracellular Ca2+ concentration was 1.20 mM, the basal cytosolic Ca2+ activity was 162 +/- 9 nM. Stimulation with 20 mM glucose increased this Ca2+ activity by 40%. In the presence of only 0.20 mM Ca2+ or after the addition of the voltage-dependent Ca2+ -channel blocker D-600, glucose had an opposite and more prompt effect in reducing cytosolic Ca2+ by about 15%. It is concluded that an early result of glucose exposure is a lowering of the cytosolic Ca2+ activity and that this effect tends to be masked by a subsequent increase of the Ca2+ activity due to influx of Ca2+ through the voltage-dependent Ca2+ channels. |
| spellingShingle | Rorsman, P Abrahamsson, H Gylfe, E Hellman, B Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title | Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title_full | Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title_fullStr | Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title_full_unstemmed | Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title_short | Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells. |
| title_sort | dual effects of glucose on the cytosolic ca2 activity of mouse pancreatic beta cells |
| work_keys_str_mv | AT rorsmanp dualeffectsofglucoseonthecytosolicca2activityofmousepancreaticbetacells AT abrahamssonh dualeffectsofglucoseonthecytosolicca2activityofmousepancreaticbetacells AT gylfee dualeffectsofglucoseonthecytosolicca2activityofmousepancreaticbetacells AT hellmanb dualeffectsofglucoseonthecytosolicca2activityofmousepancreaticbetacells |