Mechanical design principles of a mitotic spindle
An organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a slender morphology and must elongate against compressive forces. This 'pu...
| Glavni autori: | , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
eLife Sciences Publications
2015
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| _version_ | 1826269217602666496 |
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| author | Ward, J Roque, H Antony, C Nédélec, F |
| author_facet | Ward, J Roque, H Antony, C Nédélec, F |
| author_sort | Ward, J |
| collection | OXFORD |
| description | An organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a slender morphology and must elongate against compressive forces. This 'pushing' mode of chromosome transport renders the spindle susceptible to breakage, as observed in cells with a variety of defects. Here we perform electron tomographic analyses of the spindle, which suggest that it organises a limited supply of structural components to increase its compressive strength. Structural integrity is maintained throughout the spindle's fourfold elongation by organising microtubules into a rigid transverse array, preserving correct microtubule number and dynamically rescaling microtubule length. |
| first_indexed | 2024-03-06T21:21:36Z |
| format | Journal article |
| id | oxford-uuid:41a18969-7a15-4c76-bc17-c7ca9c0970dc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T21:21:36Z |
| publishDate | 2015 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:41a18969-7a15-4c76-bc17-c7ca9c0970dc2022-03-26T14:44:50ZMechanical design principles of a mitotic spindleJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:41a18969-7a15-4c76-bc17-c7ca9c0970dcEnglishSymplectic Elements at OxfordeLife Sciences Publications2015Ward, JRoque, HAntony, CNédélec, FAn organised spindle is crucial to the fidelity of chromosome segregation, but the relationship between spindle structure and function is not well understood in any cell type. The anaphase B spindle in fission yeast has a slender morphology and must elongate against compressive forces. This 'pushing' mode of chromosome transport renders the spindle susceptible to breakage, as observed in cells with a variety of defects. Here we perform electron tomographic analyses of the spindle, which suggest that it organises a limited supply of structural components to increase its compressive strength. Structural integrity is maintained throughout the spindle's fourfold elongation by organising microtubules into a rigid transverse array, preserving correct microtubule number and dynamically rescaling microtubule length. |
| spellingShingle | Ward, J Roque, H Antony, C Nédélec, F Mechanical design principles of a mitotic spindle |
| title | Mechanical design principles of a mitotic spindle |
| title_full | Mechanical design principles of a mitotic spindle |
| title_fullStr | Mechanical design principles of a mitotic spindle |
| title_full_unstemmed | Mechanical design principles of a mitotic spindle |
| title_short | Mechanical design principles of a mitotic spindle |
| title_sort | mechanical design principles of a mitotic spindle |
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